Loss of pod set caused by drought stress is associated with water status and ABA content of reproductive structures in soybean
Loss of pod set caused by drought stress is associated with water status and ABA content of reproductive structures in soybean
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DOI：
10.1071/fp02185
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发表时间：
2003-01-01
影响因子：
3
通讯作者：
Jensen, CR
中科院分区：
文献类型：
--
作者：
Liu, FL;Andersen, MN;Jensen, CR
干旱胁迫发生在开花和早期结荚期间，会降低大豆(Glyine max L.Merr.)的结荚。结荚失败可能与干旱胁迫下大豆生殖结构中水分状况和ABA含量的变化有关。为了验证这一点，在一个环境控制的温室里进行了盆栽试验，在温室里，大豆在开花前后暴露在干旱胁迫下。在初步实验中(实验I)，在开花后-6(D1)、-4(D2)和-2(D3)至11d(DAA)停止灌溉，然后重新浇水至对照水平，直到生理成熟。记录结角率、种子产量及产量构成因素。在主要实验中(实验II)，在-11~10天内停止灌溉。在干燥周期中，在0、3、5、7和10DAA时对部分干旱植株进行重新浇水，并保持充分浇水直到生理成熟。在实验中。测定0、3、5、7、10天的水分状况、木质部汁液、叶片、花和豆荚中ABA的含量。花荚水势始终低于叶水势。干旱3天后，叶片膨大率降低，但花和豆荚膨大率保持在对照水平。与水分充足的植株相比，重度干旱(10天)植株的木质部[ABA]增加了约60倍，叶片[ABA]增加了9倍，角果[ABA]增加了6倍。在土壤干燥过程中，花荚[ABA]与木质部[ABA]和叶片[ABA]呈线性相关，表明根源ABA和/或叶ABA可能是花荚中积累的ABA的来源。在实验中。干旱胁迫对种子产量和单株种子重无明显影响，但对I、结荚数和每角粒数无明显影响。在实验中。第二，当在3-5DAA重新给干旱植株浇水时，结荚和种子产量显著下降，在此阶段以后再给干旱植株重新浇水，结果是相似的结荚。综上所述，这些结果表明，干旱诱导的水势下降和关键发育期(3-5DAA)花荚中ABA含量的增加有助于大豆结荚败育。
Drought stress occurring during flowering and early pod expansion decreases pod set in soybean (Glycine max L. Merr.). The failure of pod set may be associated with changes in water status and ABA content in soybean reproductive structures under drought stress. To test this, pot experiments in an environmentally-controlled greenhouse were conducted, in which soybeans were exposed to drought stress around anthesis. In a preliminary experiment (Expt. I), irrigation was withheld at -6 (D1), -4 (D2) and -2 (D3) to 11 days after anthesis (DAA), then the droughted plants were re-watered to control levels until physiological maturity. Pod set percentage, seed yield and yield components were recorded. In the main experiment (Expt. II), irrigation was withheld from -11 to 10 DAA. During the drying cycle, parts of the droughted plants were re-watered at 0, 3, 5, 7 and 10 DAA and kept well-watered until physiological maturity. In Expt. II, water status, ABA contents in xylem sap, leaves, flowers and pods were measured at 0, 3, 5, 7 and 10 DAA. The water potential in the flowers and pods was always lower than the leaf water potential. Turgor was decreased in leaves by drought 3 DAA, but remained at control levels in flowers and pods. Compared with well-watered plants, in severely droughted plants (10 DAA), xylem [ABA] increased about 60-fold; leaf [ABA] increased 9-fold; pod [ABA] increased 6-fold. During soil drying, flower and pod [ABA] was linearly correlated with xylem [ABA] and leaf [ABA], indicating that root-originated ABA and/or leaf ABA were the likely sources of ABA accumulated in the flowers and pods. In Expt. I, pod set and seed number per pod was unaffected by drought stress, while seed yield and individual seed weight was significantly decreased by drought. In Expt. II, significant reductions in pod set and seed yield were observed when re-watering the droughted plants at 3-5 DAA, re-watering the droughted plants later than this stage resulted in a similar pod set. Collectively, these results suggest that drought-induced decrease in water potential and increase in ABA content in flowers and pods at critical developmental stage (3-5 DAA) contribute to pod abortion in soybean.