915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53BP1 foci in human lymphocytes from hypersensitive and healthy persons
915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53BP1 foci in human lymphocytes from hypersensitive and healthy persons
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DOI：
10.1002/bem.20103
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发表时间：
2005-04-01
影响因子：
1.9
通讯作者：
Harms-Ringdahl, M
中科院分区：
文献类型：
--
作者：
Belyaev, IY;Hillert, L;Harms-Ringdahl, M
我们用全球移动通信系统(GSM)手机的微波(915 MHz，比吸收率(SAR)37 mW/kg)和工频磁场(50 Hz，15mT峰值)来研究健康受试者和报告对电磁场(EMF)过敏的人淋巴细胞的反应。将过敏和健康的供者按性别和年龄匹配，并对数据进行盲法分析。用反常粘度时间依赖性(AVTD)法检测染色质构象的变化。用原位免疫组织化学方法检测与DNA双链断裂(DSB)共定位于病灶内的53BP1蛋白。暴露在915 MHz或50 Hz的室温下，染色质显著凝结，表现为AVTD变化，这与41℃热休克的影响相似，正常受试者和超敏受试者之间的反应没有发现显著差异。915 MHz和50 Hz照射均未引起53BP1灶。相反，在这些暴露以及热休克治疗后，观察到53BP1信号的背景水平明显下降。这一下降与AVTD数据相关，可能表明由于应激诱导的染色质凝聚，53BP1与抗体的可及性降低。通过形态学改变和脉冲场凝胶电泳法(PFGE)分析DNA的凋亡片段化来确定细胞的凋亡率。50赫兹和915兆赫微波不能诱导细胞凋亡。总之，在特定的暴露条件下，50赫兹磁场和915兆赫微波在健康和超敏供者的淋巴细胞中诱导了类似的反应，这些反应与热休克诱导的应激反应相似，但不完全相同。《生物电磁学》26：173-184,2005。(C)2005年Wiley-Liss，Inc.
We used exposure to microwaves from a global system for mobile communication (GSM) mobile phone (915 MHz, specific absorption rate (SAR) 37 mW/kg) and power frequency magnetic field (50 Hz, 15 mu T peak value) to investigate the response of lymphocytes from healthy subjects and from persons reporting hypersensitivity to electromagnetic field (EMF). The hypersensitive and healthy donors were matched by gender and age and the data were analyzed blind to treatment condition. The changes in chromatin conformation were measured with the method of anomalous viscosity time dependencies (AVTD). 53BP1 protein, which has been shown to colocalize in foci with DNA double strand breaks (DSBs), was analyzed by immunostaining in situ. Exposure at room temperature to either 915 MHz or 50 Hz resulted in significant condensation of chromatin, shown as AVTD changes, which was similar to the effect of heat shock at 41 degrees C. No significant differences in responses between normal and hypersensitive subjects were detected. Neither 915 MHz nor 50 Hz exposure induced 53BP1 foci. On the contrary, a distinct decrease in background level of 53BP1 signaling was observed upon these exposures as well as after heat shock treatments. This decrease correlated with the AVTD data and may indicate decrease in accessibility of 53BP1 to antibodies because of stress-induced chromatin condensation. Apoptosis was determined by morphological changes and by apoptotic fragmentation of DNA as analyzed by pulsed-field gel electrophoresis (PFGE). No apoptosis was induced by exposure to 50 Hz and 915 MHz microwaves. In conclusion, 50 Hz magnetic field and 915 MHz microwaves under specified conditions of exposure induced comparable responses in lymphocytes from healthy and hypersensitive donors that were similar but not identical to stress response induced by heat shock. Bioelectromagnetics 26:173-184, 2005. (c) 2005 Wiley-Liss, Inc.