Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins

Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins
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重组 Reelin 和 ADAMTS-3 蛋白的表达和制备

DOI：
10.1007/978-1-4939-9698-8_8
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发表时间：
2019
期刊：
Methods Mol. Biol.
影响因子：
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通讯作者：
Hattori Mitsuharu
Hattori Mitsuharu
中科院分区：
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文献类型：
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作者：
Kohno Takao;Ogino Himari;Yamakage Yuko;Hattori Mitsuharu

文献摘要

相似文献

Reelin是一种大的分泌性蛋白质，对大脑发育和功能至关重要。Reelin通过具有血小板反应蛋白1型基序3（ADAMTS-3）的去整合素和金属蛋白酶的特异性切割负调节，所述去整合素和金属蛋白酶也从神经元分泌。很可能有其他蛋白酶可以切割Reelin。本章描述了重组Reelin和ADAMTS-3蛋白的表达和处理方案，以促进这些蛋白的研究。
Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins.