FAM122A, a new endogenous inhibitor of protein phosphatase 2A.
FAM122A, a new endogenous inhibitor of protein phosphatase 2A.
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FAM122A，一种新型内源性蛋白磷酸酶2A抑制剂
DOI：
10.18632/oncotarget.11698
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发表时间：
2016-09-27
期刊：
影响因子：
--
通讯作者：
Huang Y
中科院分区：
文献类型：
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作者：
Fan L;Liu MH;Guo M;Hu CX;Yan ZW;Chen J;Chen GQ;Huang Y
普遍表达的蛋白磷酸酶2A（PP 2A）的调节对于多种细胞功能如细胞增殖、转化和命运决定是必需的。在本研究中，我们证明了在哺乳动物中高度保守的蛋白质，命名为FAM 122 A，直接与PP 2A-A α和B55α而不是B56α亚基相互作用，并抑制PP 2A-A α/B55α/Cα复合物的磷酸酶活性。此外，FAM 122 A增强了催化亚基PP 2A-C α的降解，增加了多聚泛素化。与此一致，FAM 122 A沉默抑制，而其过表达增强细胞生长和集落形成能力。综上所述，我们确定FAM 122 A为一种新的内源性PP 2A抑制剂，其生理和病理生理意义值得进一步研究。
The regulation of the ubiquitously expressed protein phosphatase 2A (PP2A) is essential for various cellular functions such as cell proliferation, transformation, and fate determination. In this study, we demonstrate that the highly conserved protein in mammals, designated FAM122A, directly interacts with PP2A-Aα and B55α rather than B56α subunits, and inhibits the phosphatase activity of PP2A-Aα/B55α/Cα complex. Further, FAM122A potentiates the degradation of catalytic subunit PP2A-Cα with the increased poly-ubiquitination. In agreement, FAM122A silencing inhibits while its overexpression enhances cell growth and colony-forming ability. Collectively, we identify FAM122A as a new endogenous PP2A inhibitor and its physiological and pathophysiological significances warrant to be further investigated.