FABRIC ORGANIZATION OF THE SUBENDOTHELIUM OF THE HUMAN BRAIN ARTERY BY POLARIZED-LIGHT MICROSCOPY
FABRIC ORGANIZATION OF THE SUBENDOTHELIUM OF THE HUMAN BRAIN ARTERY BY POLARIZED-LIGHT MICROSCOPY
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DOI：
10.1161/01.atv.11.3.681
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发表时间：
1991-05-01
期刊：
影响因子：
--
通讯作者：
CANHAM, PB
中科院分区：
文献类型：
--
作者：
FINLAY, HM;DIXON, JG;CANHAM, PB
以动脉粥样硬化为特征的脑动脉内皮下增厚的两个主要双折射成分--平滑肌细胞和胶原蛋白的方向性组织被评估。对16例尸检标本的33条动脉分别施加30、60、110和200 mm Hg的压力，切片厚度为7 mm-m，并用浸银染色以增强组织双折射。这项研究的目的是研究肌肉组织，但也包括细胞中的胶原，因为这两个组织共同排列，以及它们在偏光显微镜下相似的染色特性。在所有压力下，双折射内侧织物都是圆周取向的，33个截面的平均偏离圆周1.4度，平均圆形标准偏差3.5度，因此显示出显著的一致性。相反，内皮下层在厚度和组织结构上都有很大的变化。许多动脉没有可测量的内皮下层，而另一些则高达100%，有些动脉粥样硬化病变高达中层宽度的300%。内皮下层的测量显示，组织呈螺旋状排列，通常分为不同的区域，左右旋螺旋成分平衡，在邻近管腔的层中通常具有较低的螺距角度。单个内皮下层内的平均圆形标准差为14.5度。
The thickened subendothelium of brain arteries that is characteristic of atherosclerosis was assessed for the directional organization of the two main birefringent components, smooth muscle cells and collagen. Thirty-three arteries from 16 autopsy cases were pressure fixed at 30, 60, 110, and 200 mm Hg, sectioned at a thickness of 7-mu-m, and stained with silver impregnation to enhance tissue birefringence. The intended focus of the study was on muscle organization, but it also included the collagen among the cells because of the coalignment of the two tissues and their similar staining properties for polarized-light microscopy. The birefringent medial fabric at all pressures was circumferentially oriented, with a mean deviation of the 33 sections of 1.4-degrees from circumferential with an average circular standard deviation of 3.5-degrees, thereby showing remarkable coherence. In contrast, the subendothelium showed great variability both in thickness and in organization. Many arteries had no measurable subendothelium, and others had as much as 100%, with some atherosclerotic lesions as much as 300% of the medial width. Measurements from the subendothelium revealed a helical arrangement of tissue, often divided into separate regions, with a balance of left- and right-handed helical components and generally with lower pitch angles in the layers adjacent to the lumen. The average circular standard deviation within individual subendothelial layers was 14.5-degrees.