Metabolomic profile of umbilical cord blood plasma from early and late intrauterine growth restricted (IUGR) neonates with and without signs of brain vasodilation.
Metabolomic profile of umbilical cord blood plasma from early and late intrauterine growth restricted (IUGR) neonates with and without signs of brain vasodilation.
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DOI：
10.1371/journal.pone.0080121
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gratacós E
中科院分区：
文献类型：
--
作者：
Sanz-Cortés M;Carbajo RJ;Crispi F;Figueras F;Pineda-Lucena A;Gratacós E
目的：通过核磁共振波谱对诊断为不同临床类型宫内生长受限(IUGR)的新生儿脐静脉血浆代谢变化进行全谱分析。本研究包括23例早期IUGR患者和23例配对的足月足月(AGA)对照组，56例晚期IUGR患者和56例配对的AGA。早期胎儿宫内发育迟缓定义为出生体重<10公分位数、脐动脉(UA)多普勒异常、分娩<35周。晚期IUGR定义为出生体重10百分位数，UA多普勒正常，分娩35周。将18例血管扩张(VD)晚期IUGR胎儿和38例非VD晚期IUGR胎儿分为两组。所有AGA患者的出生体重均为10百分位数。获得分娩时脐静脉采血的1H核磁共振代谢组学。多变量统计分析确定了几种代谢物，允许区分不同的IUGR亚组，并从核磁共振数据中量化了它们的相对水平。基于核磁共振的分析显示，IUGR早期和晚期样本的不饱和脂类和极低密度脂蛋白水平升高，早期IUGR的血糖下降和丙酮水平上升。在IUGR晚期，血糖下降和丙酮水平升高的趋势并不显著，当考虑到VD和非VD亚组时，这一趋势遵循严重程度梯度。在氨基酸及其衍生物方面，早期IUGR的谷氨酰胺和肌酸水平显著升高，而苯丙氨酸和酪氨酸的含量在VD早期和晚期IUGR中显著降低。在IUGR晚期样本中，Valine和Leucine降低。所有临床亚型IUGR的胆碱水平均降低。IUGR与一种独特的代谢谱无关，但在研究和临床实践中使用的不同临床亚群中存在重要的变化。这些结果可能有助于全面描述不同IUGR亚组下的特异性改变。
To characterize via NMR spectroscopy the full spectrum of metabolic changes in umbilical vein blood plasma of newborns diagnosed with different clinical forms of intrauterine growth restriction (IUGR). 23 early IUGR cases and matched 23 adequate-for-gestational-age (AGA) controls and 56 late IUGR cases with 56 matched AGAs were included in this study. Early IUGR was defined as a birth weight <10th centile, abnormal umbilical artery (UA) Doppler and delivery <35 weeks. Late IUGR was defined as a birth weight <10th centile with normal UA Doppler and delivery >35 weeks. This group was subdivided in 18 vasodilated (VD) and 38 non-VD late IUGR fetuses. All AGA patients had a birth weight >10th centile. 1H nuclear magnetic resonance (NMR) metabolomics of the blood samples collected from the umbilical vein at delivery was obtained. Multivariate statistical analysis identified several metabolites that allowed the discrimination between the different IUGR subgroups, and their comparative levels were quantified from the NMR data. The NMR-based analysis showed increased unsaturated lipids and VLDL levels in both early and late IUGR samples, decreased glucose and increased acetone levels in early IUGR. Non-significant trends for decreased glucose and increased acetone levels were present in late IUGR, which followed a severity gradient when the VD and non-VD subgroups were considered. Regarding amino acids and derivatives, early IUGR showed significantly increased glutamine and creatine levels, whereas the amounts of phenylalanine and tyrosine were decreased in early and late-VD IUGR samples. Valine and leucine were decreased in late IUGR samples. Choline levels were decreased in all clinical subforms of IUGR. IUGR is not associated with a unique metabolic profile, but important changes are present in different clinical subsets used in research and clinical practice. These results may help in characterizing comprehensively specific alterations underlying different IUGR subsets.
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影响因子：
9.8
作者：
Crispi, Fatima;Figueras, Francesc;Gratacos, Eduard
通讯作者：
Gratacos, Eduard
影响因子：
7.2
作者：
Cruz-Martinez, Rogelio;Figueras, Francesc;Gratacos, Eduard
通讯作者：
Gratacos, Eduard
影响因子：
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作者：
Barton, Richard H.;Waterman, Daniel;Lindon, John C.
通讯作者：
Lindon, John C.
影响因子：
2.2
作者：
Cruz-Lemini, Monica;Crispi, Fatima;Gratacos, Eduard
通讯作者：
Gratacos, Eduard
DOI：
10.3109/00016349.2010.501370
发表时间：
2010-09-01
影响因子：
4.3
作者：
Engineer, Neelam;Kumar, Sailesh
通讯作者：
Kumar, Sailesh