Development of a system to sensitively and specifically visualize-fos mRNA in living cells using bispyrene-modified RNA probes
Development of a system to sensitively and specifically visualize-fos mRNA in living cells using bispyrene-modified RNA probes
复制标题
开发使用双芘修饰的 RNA 探针灵敏且特异性地可视化活细胞中 fos mRNA 的系统
DOI：
10.1039/c0cc04639f
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发表时间：
2011
影响因子：
4.9
通讯作者：
Murakami A.
中科院分区：
文献类型：
--
作者：
Waki R.;Yamayoshi A.;Kobori A.;Murakami A.
采用双芘修饰的2’- o -甲基RNA探针（OMUpy2）或硫代磷酸酯修饰的OMUpy2 (OMUpy2- s)，对固定细胞和活细胞中的c-fosmRNA进行了原位可视化，具有RNA特异性和高灵敏度，并成功开发了c-fosmRNA的延时成像系统。
In situ visualization of c-fosmRNA was shown both in fixed cells and in living cells using bispyrene-modified 2′-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fosmRNA was successfully developed.