Identification and characterization of a fluorescent flagellar protein from the brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae):: A flavoprotein homologous to Old Yellow Enzyme
Identification and characterization of a fluorescent flagellar protein from the brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae):: A flavoprotein homologous to Old Yellow Enzyme
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DOI：
10.1080/09670260500063193
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发表时间：
2005-05-01
影响因子：
2.4
通讯作者：
Murakami, A
中科院分区：
文献类型：
--
作者：
Fujita, S;Iseki, M;Murakami, A
棕色花管藻游动孢子的后鞭毛发出明亮的绿色自发荧光。为了鉴定荧光鞭毛物质，我们从游动孢子中分离出鞭毛，并通过阴离子交换和凝胶过滤色谱法部分纯化了一种黄素蛋白。荧光光谱和色谱分析表明，黄素蛋白的表观分子量为41 kDa和非共价结合的黄素monopolytide作为发色团。根据该蛋白的部分氨基酸序列，克隆了41-kDa黄素蛋白的cDNA并测序。推导的氨基酸序列与蛋白细菌、酵母和维管植物中的老黄酶家族的氨基酸序列同源。
The posterior flagellum of the zoospore of the brown alga Scytosiphon lomentaria exhibits bright green autofluorescence. To identify the fluorescent flagellar substance(s), we isolated flagella from zoospores and partially purified a flavoprotein by anion-exchange and gel-filtration chromatography. Spectrofluorometric and chromatographic analyses showed that the flavoprotein had an apparent molecular mass of 41 kDa and a non-covalently bound flavin mononucleotide as a chromophore. Based on partial amino acid sequences of the protein, a cDNA of the 41-kDa flavoprotein was cloned and sequenced. The deduced amino acid sequence of the cDNA was homologous to that of the Old Yellow Enzyme family distributed in proteobacteria, yeasts and vascular plants.