The effect of piliation and exoproduct expression on the adherence of Pseudomonas aeruginosa to respiratory epithelial monolayers.
The effect of piliation and exoproduct expression on the adherence of Pseudomonas aeruginosa to respiratory epithelial monolayers.
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纤毛和外产物表达对铜绿假单胞菌粘附于呼吸道上皮单层的影响。
DOI：
10.1093/infdis/161.3.541
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Prince,A
中科院分区：
文献类型：
--
作者：
Saiman,L;Ishimoto,K;Lory,S;Prince,A
研究了铜绿假单胞菌与已知菌毛蛋白 DNA 序列的粘附特性。极地菌毛显然有助于粘附，因为 35S 标记的菌毛阳性 (Pil+) 菌株与牛气管上皮单层的结合明显多于菌毛阴性 (Pil−) 突变体 (P< .05)，并且略高于超菌毛菌株。通过基因替换构建的 pil 突变型 PAK/NP 表现出低水平的附着（3.8% 的接种物粘附，而野生型菌株 PAK 为 7%），表明其他粘附素具有功能。 Pil−、鞭毛阴性菌株 PAO1150.l 结合最少（0.3% 的接种物粘附），证实了运动在结合过程中的重要性。菌株P1和PAl244的菌毛蛋白序列实际上是相同的，尽管P1的结合比PA1244多三倍。 P1 产生的蛋白酶比 PA1244 多 1 倍，并且通过转座子诱变分离的 P1 蛋白酶阴性突变体具有与 PA1244 相当的结合力。在来自磷酸盐限制培养物的细菌上清液的存在下，PAO1的粘附增加了60％，并且与上清液中的磷脂酶C活性相关。因此，可能需要几种假单胞菌基因的表达来促进与上皮表面的有效结合。
The adherence properties ofPseudomonas aeruginosastrains with known pilin DNA sequences were studied. A polar pilus clearly contributed to adherence, as 35S-labeled pilus-positive (Pil+) strains bound significantly more to bovine trachea epithelial monolayers than did pilus-negative (Pil−) mutants (P< .05) and minimally more than hyperpiliated strains. A pil−mutant PAK/NP, constructed by gene replacement, demonstrated low levels of attachment (3.8% of the inoculum adherent compared with 7% for the wild-type strain PAK), suggesting that other adhesins are functional. The Pil−, flagellum-negative strain PAO1150.l bound the least (0.3% of the inoculum adherent), confirming the importance ofmotility in the binding process. The pilin sequences of strains P1 and PAl244 were virtually identical, although P1 bound threefold more than did PA1244. P1 produced lo-fold more proteinase than did PA1244, and a proteinase-negative mutant of P1, isolated by transposon mutagenesis, had binding equivalent to that of PA1244. The adherence of PAOl was increased 60% in the presence of bacterial supernatants from phosphatelimited cultures and correlated with phospholipase C activity in the supernatant. Thus, the expression of severalPseudomonasgenes may be required to promote efficient binding to epithelial surfaces.