Atrial natriuretic factor receptor on cultured Leydig tumor cells: ligand binding and photoaffinity labeling.
Atrial natriuretic factor receptor on cultured Leydig tumor cells: ligand binding and photoaffinity labeling.
复制标题
培养的 Leydig 肿瘤细胞上的心房钠尿因子受体：配体结合和光亲和标记。
DOI：
10.1021/bi00374a022
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Misono,KS
中科院分区：
文献类型：
--
作者：
Pandey,KN;Inagami,T;Misono,KS
材料和方法 材料。具有序列 Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Ile-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr 的大鼠 ANF IV（Misono 等人，1984a）通过固相方法合成（Su-giyama 等人）等，1984）。类似地合成代表上述序列的从残基2到残基22的序列的Atriopeptin I (Currie等，1984)。牛黄体生成素（bLH）和人绒毛膜促性腺激素（hCG）是国家垂体管理局的礼物。血管紧张素 I、II 和 III 购自 Peninsula Laboratories, Inc.（贝尔蒙特，CA）。组织培养用品购自 Gibco Laboratories（纽约州格兰德岛），电泳材料购自 Bio-Rad（加利福尼亚州里士满）。琥珀酰亚胺基 4-叠氮基苯甲酸酯和三乙胺来自
Materials and Methods Materials. Rat ANF IV (Misono et al., 1984a) with the sequence Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Ile-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr was synthesized by the solid-phase method as described (Su-giyama et al., 1984). Atriopeptin I (Currie et al., 1984), representing the sequence from residue 2 through residue 22 of the abovesequence, was synthesized similarly. Bovine luteinizing hormone (bLH) and human chorionic gonadotropin (hCG) were gifts from the National Pituitary Agency. An-giotensins I, II, and III were purchased from Peninsula Laboratories, Inc.(Belmont, CA). Tissue culture supplies were obtained from Gibco Laboratories (Grand Island, NY) and electrophoresis materials from Bio-Rad (Richmond, CA). Succinimido 4-azidobenzoate and triethylamine were from