How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment
How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment
复制标题
DOI：
10.1021/acs.jchemed.0c00517
复制
发表时间：
2020-12-08
影响因子：
3
通讯作者：
Hunnicutt, Sally
中科院分区：
文献类型：
--
作者：
Cole, Renee S.;Muniz, Marc;Hunnicutt, Sally
在这个引导探究实验中，学生从苹果中提取儿茶酚酶来催化儿茶酚的氧化。他们利用产生的对二苯甲酮的紫外-可见吸光度来跟踪反应，以确定Michaelis-Menten动力学参数。学生在一个有组织的框架内进行实验选择，如选择苹果品种、反应混合物的pH值和反应抑制剂。该实验已在多所大学大小招生的物理化学实验课程中进行了测试。我们描述了实验及其在同步和异步教学课程中的实现。
In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV-vis absorbance of the p-benzophenone produced to determine the Michaelis-Menten kinetic parameters. Students make selected experimental choices within a structured framework such as selecting the apple varietal, the pH of the reaction mixture, and the reaction inhibitor. The experiment has been tested at multiple universities in physical chemistry laboratory courses with both large and small enrollments. We describe the experiment and its implementation in both synchronously and asynchronously taught courses.