Subcellular distribution of envoplakin and periplakin: insights into their role as precursors of the epidermal cornified envelope.
Subcellular distribution of envoplakin and periplakin: insights into their role as precursors of the epidermal cornified envelope.
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DOI：
10.1083/jcb.151.3.573
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发表时间：
2000-10-30
期刊：
影响因子：
--
通讯作者：
Watt FM
中科院分区：
文献类型：
--
作者：
DiColandrea T;Karashima T;Määttä A;Watt FM
Envoplakin 和 periplakin 是两种 plakin，它们是表皮角质化包膜的前体。我们通过转染原代人角质形成细胞和其他细胞来研究它们的分布和相互作用。全长周斑蛋白定位于桥粒、桥粒间质膜和中间丝。全长包膜蛋白也定位于桥粒，但主要积累在与相关中间丝一起的核和细胞质聚集体中。聚集需要 envoplakin rod 结构域，并且 periplakin rod 结构域对于将 envoplakin 重新分配到桥粒和细胞骨架来说是必要且充分的，这证实了早期关于蛋白质可以异二聚化的预测。每种蛋白质的接头结构域是中间丝缔合所必需的。与桥粒斑蛋白的 NH2 末端一样，周斑蛋白的 NH2 末端也位于桥粒上；然而，此外，periplakin NH2 末端与皮质肌动蛋白相关，在细胞表面微绒毛处积累。当用肌动蛋白破坏药物 Latrunculin B 处理角质形成细胞时，内源性周斑蛋白从微绒毛中重新分布。我们提出，虽然 envoplakin 和 periplakin 可以独立定位于桥粒，但 envoplakin 在桥粒间质膜上的分布取决于与 periplakin 的异二聚化，因此 periplakin 的 NH2 末端在形成角质化包膜所在的支架方面发挥着关键作用。组装。
Envoplakin and periplakin are two plakins that are precursors of the epidermal cornified envelope. We studied their distribution and interactions by transfection of primary human keratinocytes and other cells. Full-length periplakin localized to desmosomes, the interdesmosomal plasma membrane and intermediate filaments. Full length envoplakin also localized to desmosomes, but mainly accumulated in nuclear and cytoplasmic aggregates with associated intermediate filaments. The envoplakin rod domain was required for aggregation and the periplakin rod domain was necessary and sufficient to redistribute envoplakin to desmosomes and the cytoskeleton, confirming earlier predictions that the proteins can heterodimerize. The linker domain of each protein was required for intermediate filament association. Like the NH2 terminus of desmoplakin, that of periplakin localized to desmosomes; however, in addition, the periplakin NH2 terminus accumulated at cell surface microvilli in association with cortical actin. Endogenous periplakin was redistributed from microvilli when keratinocytes were treated with the actin disrupting drug Latrunculin B. We propose that whereas envoplakin and periplakin can localize independently to desmosomes, the distribution of envoplakin at the interdesmosomal plasma membrane depends on heterodimerization with periplakin and that the NH2 terminus of periplakin therefore plays a key role in forming the scaffold on which the cornified envelope is assembled.
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影响因子：
7.8
作者：
Geerts, D;Fontao, L;Nievers, M G;Schaapveld, R Q;Purkis, P E;Wheeler, G N;Lane, E B;Leigh, I M;Sonnenberg, A
通讯作者：
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影响因子：
7.8
作者：
Kowalczyk, AP;Bornslaeger, EA;Borgwardt, JE;Palka, HL;Dhaliwal, AS;Corcoran, CM;Denning, MF;Green, KJ
通讯作者：
Green, KJ
DOI：
10.1083/jcb.149.1.195
发表时间：
2000-04-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Karakesisoglou I;Yang Y;Fuchs E
通讯作者：
Fuchs E
影响因子：
7.8
作者：
Hatzfeld, M;Haffner, C;Vinzens, U
通讯作者：
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影响因子：
4.8
作者：
Kowalczyk, AP;Hatzfeld, M;Green, KJ
通讯作者：
Green, KJ