Single-Embryo Metabolomics and Systematic Prediction of Developmental Stage in Zebrafish
Single-Embryo Metabolomics and Systematic Prediction of Developmental Stage in Zebrafish
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DOI：
10.5560/znc.2011.66c0191
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Fukusaki, Eiichiro
中科院分区：
文献类型：
--
作者：
Hayashi, Shunsuke;Yoshida, Mako;Fukusaki, Eiichiro
代谢物是生物体中基因表达的最终产物，与生物体的发育和生长密切相关。因此，代谢分析是了解细胞、组织和个体生物体中发生的事件的潜在重要工具。在这里，我们提出了一种使用“单胚胎”的新型代谢组非目标指纹来预测斑马鱼胚胎发育阶段的方法。通过这种方法，我们观察了不同温度下的发育速度。我们的结果表明，这种方法使我们能够在单胚胎表达最终表型之前分析其状况或区分其基因型。
Metabolites, the end products of gene expression in living organisms, are tightly correlated with an organism's development and growth. Thus, metabolic profiling is a potentially important tool for understanding the events that have occurred in cells, tissues, and individual organisms. Here, we present a method for predicting the developmental stage of zebrafish embryos using novel metabolomic non-target fingerprints of "single-embryos". With this method, we observed the rate of development at different temperatures. Our results suggest that this method allows us to analyse the condition, or distinguish the genotype, of single-embryos before expression of their ultimate phenotype.