Arabidopsis SNAREs SYP61 and SYP121 Coordinate the Trafficking of Plasma Membrane Aquaporin PIP2;7 to Modulate the Cell Membrane Water Permeability
Arabidopsis SNAREs SYP61 and SYP121 Coordinate the Trafficking of Plasma Membrane Aquaporin PIP2;7 to Modulate the Cell Membrane Water Permeability
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DOI：
10.1105/tpc.114.127159
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发表时间：
2014-07-01
期刊：
影响因子：
11.6
通讯作者：
Chaumont, Francois
中科院分区：
文献类型：
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作者：
Hachez, Charles;Laloux, Timothee;Chaumont, Francois
植物质膜内源蛋白(PIP)是促进水和小分子中性溶质在生物膜中被动运动的水通道蛋白。在这里，我们报告了PIP2；7在拟南芥中高尔基体运输后涉及与两种合成素蛋白的特定相互作用，即QC-SNARE SYP61和Qa-SNARE SYP121，PIP2；7的适当转运取决于这两个SNARs的活性，并且SNARs共定位和物理相互作用。这些发现表明了SYP61和SYP121的重要作用，可能形成了一种陷阱复合体。我们的数据支持一个模型，在该模型中，特定的SNARE蛋白和PIP水通道蛋白之间的直接相互作用调节了它们在高尔基体后的运输，从而有助于微调质膜的水通透性。
Plant plasma membrane intrinsic proteins (PIPs) are aquaporins that facilitate the passive movement of water and small neutral solutes through biological membranes. Here, we report that post-Golgi trafficking of PIP2;7 in Arabidopsis thaliana involves specific interactions with two syntaxin proteins, namely, the Qc-SNARE SYP61 and the Qa-SNARE SYP121, that the proper delivery of PIP2;7 to the plasma membrane depends on the activity of the two SNAREs, and that the SNAREs colocalize and physically interact. These findings are indicative of an important role for SYP61 and SYP121, possibly forming a SNARE complex. Our data support a model in which direct interactions between specific SNARE proteins and PIP aquaporins modulate their post-Golgi trafficking and thus contribute to the fine-tuning of the water permeability of the plasma membrane.