Human alveolar lining material and antibacterial defenses.
Human alveolar lining material and antibacterial defenses.
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人类肺泡衬里材料和抗菌防御。
DOI：
10.1164/arrd.1986.133.1.136
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Lawrence Ec
中科院分区：
文献类型：
--
作者：
Steinn Jonsson;Daniel M. Musher;Allen Goree;Lawrence Ec
为了研究人肺泡衬里材料 (ALM) 可能的抗菌特性，我们通过健康不吸烟者的支气管肺泡灌洗获得了 ALM 和肺泡巨噬细胞 (PAM)。通过离心或微孔过滤分离肺泡内衬材料；电子显微镜显示层状体，脂质分析显示 98% 的脂质部分是磷脂。没有检测到游离脂肪酸。肺炎链球菌和不可分型流感嗜血杆菌 (NTHI) 在 PBS 中自发死亡，90 分钟内的平均死亡率分别为 log10 0.75 和 0.95；添加 ALM 似乎发挥了轻微的保护作用，并且在较高浓度下支持 NTHI 的复制。当 ALM 存在时，PAM 对细菌的摄取没有差异。吞噬的 NTHI 在 60 分钟内被带有或不带有 ALM 的 PAM 快速完全杀死。与 ALM 存在相比，单独使用 PAM 杀死的金黄色葡萄球菌比例更大。因此，来自健康人类的肺泡衬里材料似乎对宿主防御这些细菌没有明显的影响。我们的结果与早期使用大鼠 ALM 进行的研究结果之间的差异可能与 ALM 成分的种间差异有关。
To investigate the possible antibacterial properties of human alveolar lining material (ALM), we obtained ALM and pulmonary alveolar macrophages (PAM) by bronchoalveolar lavage of healthy nonsmokers. Alveolar lining material was isolated by centrifugation or micropore filtration; electron microscopy revealed lamellar bodies, and lipid analysis showed that 98% of the lipid fraction was phospholipid. No free fatty acids were detected. Streptococcus pneumoniae and non-typable Haemophilus influenzae (NTHI) died spontaneously in PBS at a mean rate of log10 0.75 and 0.95 in 90 min, respectively; the addition of ALM appeared to exert a slight protective effect, and at higher concentrations supported replication of NTHI. There was no difference in the uptake of the bacteria by PAM when ALM was present. Phagocytosed NTHI were killed rapidly and completely within 60 min by PAM with or without ALM. A greater proportion of S. aureus were killed by PAM alone than in the presence of ALM. Alveolar lining material from healthy humans thus appears to have no demonstrable effect on host defense against these bacteria. The differences between our results and those of earlier studies using ALM from rats may relate to interspecies differences in the composition of ALM.