Comparison of Stress‐induced Changes in Noradrenergic and Serotonergic Neurons in the Rat Hippocampus Using Microdialysis
Comparison of Stress‐induced Changes in Noradrenergic and Serotonergic Neurons in the Rat Hippocampus Using Microdialysis
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使用微透析比较应激引起的大鼠海马去甲肾上腺素能和血清素能神经元的变化
DOI：
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发表时间：
1994
影响因子：
3.4
通讯作者：
M. Fillenz
中科院分区：
文献类型：
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作者：
A. Vahabzadeh;M. Fillenz
在自由活动的大鼠中，利用微透析技术比较了应激对海马肾上腺素能和去甲肾上腺素能投射的影响。在存在各自的摄取阻断剂的情况下，测量了5-羟色胺（5-HT）、去甲肾上腺素及其代谢产物5-羟基吲哚乙酸（5-HIAA）和3，4-二羟基苯乙酸（DOPAC）的应激诱导变化。采用河豚毒素局部注射和Cd ~（2+）替代Ca ~（2+）的方法检测对脉冲流量的依赖性。夹尾5分钟或束缚应激10分钟可增加5-HT、5-HIAA、去甲肾上腺素和DOPAC水平。皮下注射生理盐水导致5-HT和DOPAC增加，但未导致去甲肾上腺素或5-HIAA增加。尽管α2肾上腺素受体激动剂和拮抗剂导致去甲肾上腺素和DOPAC的基线值发生变化，但它们对应激诱导的变化影响很小或没有影响。脉冲交通的取消和压力的增强对递质的影响大于对代谢物水平的影响。尽管去甲肾上腺素能和肾上腺素能通路对应激的反应显示出许多相似之处，但有证据表明它们通过不同的通路激活。
The effects of stress on the serotonergic and noradrenergic projection to the hippocampus were compared in freely moving rats using microdialysis. Stress‐induced changes in 5‐hydroxytryptamine (5‐HT), noradrenaline and their metabolites 5‐hydroxyindoleacetic acid (5‐HIAA) and 3,4‐dihydroxyphenylacetic acid (DOPAC) were measured in the presence of their respective uptake blockers. Local infusion of tetrodotoxin and replacement of Ca2+ with Cd2+ were used to test dependence on impulse traffic. A 5 min tail pinch or 10 min restraint stress increased 5‐HT, 5‐HIAA, noradrenaline and DOPAC levels. A subcutaneous saline injection produced an increase in 5‐HT and DOPAC but not noradrenaline or 5‐HIAA. Although α2 adrenoceptor agonists and antagonists produced changes in the baseline values of noradrenaline and DOPAC, they had little or no effect on stress‐induced changes. Both the abolition of impulse traffic and its enhancement by stress had a greater effect on transmitter than on metabolite levels. Although the responses to stress of the noradrenergic and serotonergic pathway showed many similarities, there was evidence for their activation by separate pathways.