Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor.
Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor.
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合成磷酸肽免疫原产生针对 c-erbB-2 受体的激活特异性抗体。
DOI：
10.1073/pnas.89.21.10435
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发表时间：
1992
影响因子：
11.1
通讯作者：
Stiles,CD
中科院分区：
文献类型：
--
作者：
Epstein,RJ;Druker,BJ;Roberts,TM;Stiles,CD
我们用合成的磷酸肽接种兔子，复制了c-erbB-2原癌基因产物的一个主要自磷酸化位点。兔产生的抗血清，反向免疫亲和纯化后，选择性地识别erbB-2蛋白在其酶活性的配置。这些抗磷酸肽抗血清鉴定了erbB-2阳性人细胞系的一个亚群，其中该蛋白质作为酪氨酸激酶具有组成型活性。合成的磷酸肽掺入信息蛋白磷酸化位点可能证明可用于产生指示另外的酪氨酸激酶和可能在丝氨酸和苏氨酸残基上磷酸化的其他蛋白的活化状态的抗体。
We inoculated rabbits with synthetic phosphopeptides, duplicating a major autophosphorylation site of the c-erbB-2 protooncogene product. The rabbits produced antisera that, after reverse immunoaffinity purification, selectively recognize the erbB-2 protein in its enzymatically active configuration. These anti-phosphopeptide antisera identify a subset of erbB-2-positive human cell lines wherein the protein is constitutively active as a tyrosine kinase. Synthetic phosphopeptides incorporating informative protein phosphorylation sites may prove useful for generating antibodies that indicate the activation state of additional tyrosine kinases and perhaps other proteins phosphorylated on serine and threonine residues.