Orientation of the agarose gel matrix in pulsed electric fields.
Orientation of the agarose gel matrix in pulsed electric fields.
复制标题
脉冲电场中琼脂糖凝胶基质的取向。
DOI：
10.1093/nar/17.4.1537
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发表时间：
1989
影响因子：
14.9
通讯作者：
Stellwagen,NC
中科院分区：
文献类型：
--
作者：
Stellwagen,J;Stellwagen,NC
采用瞬态电双折射技术研究了脉冲电场对琼脂糖凝胶基质取向的影响。在所有电场强度下观察凝胶的取向。当10-100 V/cm的脉冲作用于1%的凝胶0.5-2秒时，观察到非常缓慢的时间依赖性效应，表明基体的畴被电场定向。当外加电场方向反转时，双折射符号反转，表明电场反转后畴倾向于垂直方向定向。凝胶电泳理论将需要纳入基质的取向，以便提供琼脂糖凝胶电泳的完整解释。
The technique of transient electric birefringence was used to investigate the effect of pulsed electric fields on the orientation of the agarose gel matrix. Orientation of the gel was observed at all electric field strengths. Very slow, time-dependent effects were observed when pulses of 10–100 V/cm were applied to 1% gels for 0.5–2 seconds, indicating that domains of the matrix were being oriented by the electric field. The sign of the birefringence reversed when the direction of the applied electric field was reversed, indicating that the domains tend to orient in the perpendicular direction after field reversal. Theories of gel electrophoresis will need to incorporate the orientation of the matrix in order to provide a complete explanation of electrophoresis in agarose gels.