Topographic distribution of output neurons in cerebellar nuclei and cortex to somatotopic map of primary motor cortex
Topographic distribution of output neurons in cerebellar nuclei and cortex to somatotopic map of primary motor cortex
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DOI：
10.1111/j.1460-9568.2007.05482.x
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发表时间：
2007-04-01
影响因子：
3.4
通讯作者：
Takada, Masahiko
中科院分区：
文献类型：
--
作者：
Lu, Xiaofeng;Miyachi, Shigehiro;Takada, Masahiko
为了研究小脑的体位组织，我们利用狂犬病病毒的逆行跨神经元运输分析了初级运动皮质(MI)的多突触输入。在近侧前肢、远侧前肢和后肢注射狂犬病病毒后3d，在小脑深部核(包括齿状核、前错位核和后错位核)标记了经丘脑的二级神经元。在糖尿病肾病中，前肢和后肢的标记主要见于背侧。后肢的标记位于前肢的吻侧，前肢近端的标记位于前肢远端的略微吻侧。在AIN中也观察到同样的吻尾排列。然而，在后错位核，MI后肢和前肢的标记在很大程度上重叠。注射后4天，小脑浦肯野细胞出现三级标记。前肢近端和远端浦肯野细胞的标记主要见于小叶IV-VI和小腿I，近侧前肢标记位于小叶IV-VI内远端前肢的吻侧。然而，在小叶III-VI内，后肢标记在前肢近端的吻侧和侧方均可见。这些结果表明，在DN和AIN中，后肢、前肢近端和前肢远端呈轮尾状排列，而小脑皮质则沿轮尾轴和外侧轴存在双重体位。
To investigate the somatotopic organization of the cerebellum, we analysed multisynaptic inputs to the primary motor cortex (MI) using retrograde transneuronal transport of rabies virus. At 3 days after rabies injections into proximal forelimb, distal forelimb and hindlimb representations of the macaque MI, second-order neurons via the thalamus were labeled in the deep cerebellar nuclei, including the dentate (DN), anterior interpositus (AIN) and posterior interpositus nuclei. In the DN, the labeling of both the forelimb and hindlimb was seen mainly in the dorsal aspect. The labeling of the hindlimb was located rostral to that of the forelimb and the labeling of the proximal forelimb was located slightly rostral to that of the distal forelimb. The same rostrocaudal arrangement was observed in the AIN. In the posterior interpositus nucleus, however, labeling from the MI hindlimb and forelimb representations largely overlapped. At the 4-day postinjection period, third-order labeling occurred in Purkinje cells of the cerebellar hemisphere. The Purkinje cell labeling from the forelimb representation, including the proximal and distal regions, was observed primarily in lobules IV-VI and crus I. The proximal forelimb labeling was both rostral and lateral to that of the distal forelimb within lobules IV-VI. However, the hindlimb labeling was seen both rostral and lateral to that of the proximal forelimb within lobules III-VI. These results indicate that the hindlimb, proximal forelimb and distal forelimb are arranged rostrocaudally in the DN and AIN, whereas there is dual somatotopy along the rostrocaudal and lateromedial axes in the cerebellar cortex.