Quantitative 1H NMR spectroscopy of blood plasma metabolites
Quantitative 1H NMR spectroscopy of blood plasma metabolites
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DOI：
10.1021/ac020782
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发表时间：
2003-05-01
影响因子：
7.4
通讯作者：
Behar, KL
中科院分区：
文献类型：
--
作者：
de Graaf, RA;Behar, KL
通过质子NMR光谱法对血浆代谢物进行绝对定量由于存在源自血清大分子和脂蛋白的基线和宽共振而变得复杂。本文提出了一种血浆质子核磁共振谱的简化方法。利用平移扩散系数的巨大差异，结合扩散敏化质子NMR光谱，实现了血清大分子和代谢物的完全分离。血浆代谢物的浓度可以通过使用甲酸盐作为内部浓度参考来定量。结果与超滤，分离大分子和代谢物的传统方法得到的结果进行了比较，并证明了这两种方法之间的良好的相关性。扩散敏化NMR光谱的一般性质允许在广泛的生物流体上应用。
The absolute quantification of blood plasma metabolites by proton NMR spectroscopy is complicated by the presence of a baseline and broad resonances originating from serum macromolecules and lipoproteins. A method for spectral simplification of proton NMR spectra of blood plasma is presented. Serum macromolecules and metabolites are completely separated by utilizing the large difference in translational diffusion coefficients in combination with diffusion-sensitized proton NMR spectroscopy. The concentration of blood plasma metabolites can be quantified by using formate as an internal concentration reference. The results are compared with those obtained with ultrafiltration, a traditional method for separating macromolecules and metabolites, and demonstrate an excellent correlation between the two methods. The general nature of diffusion-sensitized NMR spectroscopy allows application on a wide range of biological fluids.