DNA methylation profiles in primary cutaneous melanomas are associated with clinically significant pathologic features.
DNA methylation profiles in primary cutaneous melanomas are associated with clinically significant pathologic features.
复制标题
原发性皮肤黑色素瘤中的 DNA 甲基化谱与临床显着的病理特征相关。
DOI：
10.1111/pcmr.12289
复制
发表时间：
2014
影响因子：
4.3
通讯作者：
Conway,Kathleen
中科院分区：
文献类型：
--
作者：
Thomas,NancyE;Slater,NathanielA;Edmiston,SharonN;Zhou,Xin;Kuan,Pei-Fen;Groben,PamelaA;Carson,CraigC;Hao,Honglin;Parrish,Eloise;Moschos,StergiosJ;Berwick,Marianne;Ollila,DavidW;Conway,Kathleen
DNA methylation studies have elucidated a methylation signature distinguishing primary melanomas from benign nevi and provided new insights about genes that may be important in melanoma development. However, it is unclear whether methylation differences among primary melanomas are related to tumor pathologic features with known clinical significance. We utilized the Illumina GoldenGate Cancer Panel array to investigate the methylation profiles of 47 primary cutaneous melanomas. Arraywide methylation patterns revealed a positive association of methylation with Breslow thickness and mutatedBRAF, a negative association with mitotic rate, and a weak association with ulceration. Hierarchical clustering on CpG sites exhibiting the most variable methylation (n = 235) divided the melanoma samples into three clusters, including a highly methylated cluster that was positively associated with Breslow thickness and an intermediately methylated cluster associated with Breslow thickness and mitotic rate. Our findings provide support for the existence of methylation‐defined subsets in melanomas with increased methylation associated with Breslow thickness.
