Erythroid differentiation and poly(ADP-ribose) synthesis in Friend leukemia cells.
Erythroid differentiation and poly(ADP-ribose) synthesis in Friend leukemia cells.
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Friend 白血病细胞中的红系分化和聚（ADP-核糖）合成。
DOI：
10.20772/cancersci1959.70.1_37
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
T. Ono
中科院分区：
文献类型：
--
作者：
K. Morioka;K. Tanaka;T. Nokuo;M. Ishizawa;T. Ono
烟酰胺是一种特异性的adp核糖合成酶抑制剂，在Friend红细胞白血病细胞（FLC）中被发现是一种中度的血红蛋白合成诱导剂。因此，考察了其他诱导剂如二甲亚砜（DMSO）、六亚甲基双乙酰胺（HMBA）和丁酸盐对聚adp -核糖合成的影响。经DMSO或HMBA处理的FLC细胞核中poly（adp -核糖）合成的程度在包括血红蛋白产生在内的许多表型变化之前开始下降，并在细胞进入固定期时达到未处理对照水平的30- 50%。对HMBA或DMSO没有反应的FLC变异没有表现出与这些诱导剂处理的亲本细胞一样低的聚（adp -核糖）合成活性。相比之下，丁酸盐在培养早期（6- 24小时）短暂但明显地刺激了聚腺苷核糖的合成（约50%），但在后期抑制了聚腺苷核糖的合成。诱导物的作用与细胞的生长和降解均无关。这些结果表明，聚腺苷核糖的合成水平与FLC的分化有关。
Nicotinamide, a specific inhibitor of poly(ADP-ribose) synthetase, was found to be a moderate inducer of hemoglobin synthesis in Friend erythroid leukemia cells (FLC). Therefore, the effect of other inducers, s-ch as dimethyl sulfoxide (DMSO), hexamethylene-bisacetamide (HMBA), and butyrate, on poly(ADP-ribose) synthesis was examined. The extent of poly(ADP-ribose) synthesis in nuclei of FLC treated with DMSO or HMBA began to decrease before many phenotypic changes including hemoglobin production and reached 30--50% of the level of nontreated control when the cells enter the stationary phase. FLC variants unresponsive to HMBA or DMSO did not exhibit as low an activity of poly(ADP-ribose) synthesis as their parent cells did by treatment with these inducers. In contrast, butyrate stimulated poly(ADP-ribose) synthesis transiently but distinctly (about 50%) at an early stage of culture (6--24 hr), but suppressed it at a later stage. Neither the cell growth nor degradation of poly(ADP-ribose) is correlated with the effect of inducers. These results suggest that the level of poly(ADP-ribose) synthesis is correlated with the differentiation of FLC.