Candidate chemosensory ionotropic receptors in a Lepidoptera
Candidate chemosensory ionotropic receptors in a Lepidoptera
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DOI：
10.1111/j.1365-2583.2010.01057.x
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发表时间：
2011-04-01
影响因子：
2.6
通讯作者：
Jacquin-Joly, E.
中科院分区：
文献类型：
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作者：
Olivier, V.;Monsempes, C.;Jacquin-Joly, E.
最近在黑胃果蝇中发现了一个与嗜离子性谷氨酸受体（iGluRs）相关的候选化学感觉嗜离子受体（IRs）家族。通过对夜蛾（Spodoptera littoralis）雄触角表达的序列标记文库进行Blast分析，我们鉴定出了12个编码黑腹蛾（D. melanogaster）和家蚕（Bombyx mori） ir相关蛋白的单基因。获得了它们的全长序列，并分析了它们的表达模式，表明它们在化学感觉器官中只表达或明显富集。与iGluRs相比，推断的蛋白质序列与家蚕和黑腹家蚕的IRs更相似，并且在预测的配体结合域上显示出相当大的差异；没有一种具有iGluRs中发现的三种谷氨酸相互作用残基，这表明它们的结合特异性不同。我们的研究结果表明，我们在海蛾中发现了IR化学感觉受体家族的成员，并在此报告了IR在鳞翅目中的首次表达。
A new family of candidate chemosensory ionotropic receptors (IRs) related to ionotropic glutamate receptors (iGluRs) was recently discovered in Drosophila melanogaster. Through Blast analyses of an expressed sequenced tag library prepared from male antennae of the noctuid moth Spodoptera littoralis, we identified 12 unigenes encoding proteins related to D. melanogaster and Bombyx mori IRs. Their full length sequences were obtained and the analyses of their expression patterns suggest that they were exclusively expressed or clearly enriched in chemosensory organs. The deduced protein sequences were more similar to B. mori and D. melanogaster IRs than to iGluRs and showed considerable variations in the predicted ligand-binding domains; none have the three glutamate-interacting residues found in iGluRs, suggesting different binding specificities. Our data suggest that we identified members of the insect IR chemosensory receptor family in S. littoralis and we report here the first demonstration of IR expression in Lepidoptera.