Development of Small-diameter Polyester Vascular Grafts Coated with Silk Fibroin Sponge
Development of Small-diameter Polyester Vascular Grafts Coated with Silk Fibroin Sponge
复制标题
丝素海绵涂层小直径聚酯血管的研制
DOI：
10.1080/15476278.2019.1686295
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发表时间：
2020
期刊：
影响因子：
2.3
通讯作者：
T. Asakura
中科院分区：
文献类型：
--
作者：
T. Tanaka;R. Tanaka;Y. Ogawa;Y. Takagi;T. Asakura
近年来，由于血管性心脏病患者数量的增加，对功能性小直径（< 6 mm）人工血管移植物的需求大大增加。然而，目前还没有可用于商业的小直径移植物。本研究的目的是开发一种直径< 6 mm的多孔丝素（SF）包被聚对苯二甲酸乙酯（PET）移植物。将该移植物与明胶包被PET移植物进行比较，因为后者的PET移植物直径约为6 mm，被广泛用于商业血管移植物。最初，我们以Glyc作为孔隙素制备多孔性SF[简称SF(Glyc)]，并通过双拉舍尔编织法制备PET接枝。随后，使用蛋白酶XIV在体外监测SF涂层的降解情况，并与明胶涂层PET移植物的降解情况进行比较。最后，这些移植物也被植入大鼠体内进行体内比较。降解实验中，7天后，SF被蛋白酶XIV明显消化，但移植物上的明胶仍停留在外表面。在大鼠体内植入实验中，与明胶包被PET移植相比，SF（Glyc）包被PET移植在体内快速降解，促进了对自身组织的重塑。明胶包被PET移植物可见血栓形成和内膜增生；然而，在SF（Glyc）包被的PET移植物中没有观察到这些副反应。因此，直径小于6mm的多孔SF（Glyc）包覆PET移植物可作为商业血管移植物。
In recent years, the demand for functional small-diameter (< 6 mm) artificial vascular grafts has greatly increased due to an increase in the number of patients with vascular heart disease. However, currently, there are no available commercial small-diameter grafts. The objective of this research was to develop a porous silk fibroin (SF)-coated poly(ethylene terephthalate) (PET) graft with a diameter < 6 mm. The graft was compared with a gelatin-coated PET graft because the latter PET graft with a diameter ~ 6 mm was widely used as a commercial vascular graft. Initially, porous SF was prepared using Glyc as the porogen [termed SF(Glyc)] and the PET grafts were prepared through the double-Raschel knitting method. Subsequently, the degradation of the SF coating was monitored using protease XIV in vitro and was compared with that observed in gelatin-coated PET grafts. Finally, these grafts were also implanted into rats for an in vivo comparison. In degradation experiments, after 7 days, the SF was clearly digested by protease XIV, but the gelatin on the graft was still remained at the outer surface. In implantation experiments in rats, the SF(Glyc)-coated PET graft was rapidly degradedin vivoand remodeling to self-tissues was promoted compared with the gelatin-coated PET graft. Thrombus formation and intimal hyperplasia were observed in the gelatin-coated PET graft; however, such side reactions were not observed in the SF(Glyc)-coated PET graft. Thus, the porous SF(Glyc)-coated PET graft with a small diameter < 6 mm may be useful as a commercial vascular graft.