The role of carboxylic groups in heparin-mimicking polymer-functionalized surfaces for blood compatibility: Enhanced vascular cell selectivity.
The role of carboxylic groups in heparin-mimicking polymer-functionalized surfaces for blood compatibility: Enhanced vascular cell selectivity.
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DOI：
10.1016/j.colsurfb.2021.111653
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发表时间：
2021-02
期刊：
影响因子：
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通讯作者：
Aiyang Zhang;Weibin Sun;Xinyi Liang;Xianshuang Chen;Yuepeng Li;Xiaoli Liu;Hong Chen
中科院分区：
文献类型：
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作者：
Aiyang Zhang;Weibin Sun;Xinyi Liang;Xianshuang Chen;Yuepeng Li;Xiaoli Liu;Hong Chen
血液相容性是生物医用材料永恒的话题。肝素模拟聚合物（HMPs）对血液相容性的影响已被广泛研究，特别是糖单元和磺酸基/硫酸基单元的协同作用。然而，羧基在HMP中也起重要作用。本文以4-乙烯基苯磺酸钠（SS）和2-甲基丙烯酰胺基吡喃葡萄糖（MAG）的共聚物（poly（SS-co-MAG））和聚丙烯酸（PAA）为原料，采用不同的进料比在Au表面自组装。当自组装的聚（SS-co-MAG）单独，SS和MAG的血管细胞选择性的最佳进料比为1：1（PS1 M1）;在这个比例的Au-PS1 M1表面显示出最高的人脐静脉内皮细胞（HUVECs）密度和最低的人脐静脉平滑肌细胞（HUVSMCs）密度。当PAA单独自组装（表面命名为Au-PAA）时，HUVECs和HUVSMCs的增殖均受到抑制。与单独的PS 1 M1或PAA相比，PAA和PS 1 M1以1：1的进料比（材料命名为Au-PSM/PAA-2）修饰的表面显示出对HUVECs的增强的促进作用以及对HUVSMCs的增强的抑制作用，这表明在糖和磺酸基单元存在下，血管细胞对羧基的选择性更强。
Blood compatibility is an eternal topic of biomedical materials. The effect of heparin-mimicking polymers (HMPs) on blood compatibility has been well studied, especially the synergistic effect of sugar unit and sulfonate/sulfate unit. However, carboxylic groups also play an important role in HMPs. In this work, copolymers of sodium 4-vinyl-benzenesulfonate (SS) and 2-methacrylamido glucopyranose (MAG) (poly(SS-co-MAG)) and poly(acrylate acid) (PAA) were self-assembled on Au surfaces with different feed ratios. When self-assembly of poly(SS-co-MAG) alone, the optimized feed ratio of SS and MAG for vascular cell selectivity was 1:1 (PS1M1); at this ratio the Au-PS1M1 surface showed the highest human umbilical vein endothelial cells (HUVECs) density and the lowest human umbilical vein smooth muscle cells (HUVSMCs) density. When self-assembly of PAA alone (surface designated as Au-PAA), the proliferation of both HUVECs and HUVSMCs was inhibited. Compared with either PS1M1 or PAA alone, the surfaces modified with both PAA and PS1M1 at the feed ratio of 1:1 (material designated as Au-PSM/PAA-2) showed enhanced promoting effect on HUVECs as well as enhanced inhibiting effect on HUVSMCs, indicating stronger vascular cell selectivity of carboxylic groups in the presence of sugar and sulfonate units.