Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection
Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection
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DOI：
10.1097/00007890-199803150-00027
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发表时间：
1998-03-15
期刊：
影响因子：
6.2
通讯作者：
Israël-Biet, D
中科院分区：
文献类型：
--
作者：
Charpin, JM;Valcke, J;Israël-Biet, D
背景慢性肺排斥反应（chronic lung rejection，COPD）是一种纤维增生性疾病，可导致小气道阻塞.它已成为限制肺移植受者生存的主要因素，目前缺乏肺纤维化的预测标志物，其诊断通常在纤维化过程不可逆时确定。我们采用竞争性逆转录聚合酶链反应方法定量了肺移植受者肺泡细胞中转化生长因子-β（TGF-β）的表达，TGF-β是纤维形成的关键介质。我们已经发现，与无胰腺炎的患者相比，胰腺炎患者呈现显著的TGF-β mRNA表达峰值。这些峰值出现在诊断前几个月，在诊断后2年内死亡的3例患者中有2例。我们的数据表明，肺泡细胞中TGF-β的表达可以作为慢性肺排斥反应的早期预测和预后指标。
Background Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients, Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible.Methods. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method.Results. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis.Conclusions. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.