The identification of transposon-tagged mutations in essential genes that affect cell morphology in Saccharomyces cerevisiae.
The identification of transposon-tagged mutations in essential genes that affect cell morphology in Saccharomyces cerevisiae.
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DOI：
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Kristin T. Chun;Mark G. Goebl
中科院分区：
文献类型：
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作者：
Kristin T. Chun;Mark G. Goebl
酿酒酵母通过出芽繁殖，并且许多基因是正确芽发育所必需的。这些基因中的一些突变导致细胞死亡，并具有不寻常的末端形态伸长或形状异常的芽。为了深入了解芽的发育，我们着手确定新的基因，编码蛋白质所需的适当芽形态发生。以前的研究筛选了条件突变的集合，以确定基本功能所需的基因，包括芽的形成。然而，在这样的筛选中将不会鉴定出对引起条件表型的突变的产生不敏感的基因。为了鉴定更全面的突变体集合，我们使用转座子诱变来产生大量的致死破坏突变。该集合用于鉴定209个突变体，其具有引起异常顶芽形态的破坏。这些突变体中的33个中的破坏突变确定了三个以前未表征的基因为必需的，并且突变体表型表明它们的产物在芽形态发生中的作用。
The yeast Saccharomyces cerevisiae reproduces by budding, and many genes are required for proper bud development. Mutations in some of these genes cause cells to die with an unusual terminal morphology-elongated or otherwise aberrantly shaped buds. To gain insight into bud development, we set out to identify novel genes that encode proteins required for proper bud morphogenesis. Previous studies screened collections of conditional mutations to identify genes required for essential functions, including bud formation. However, genes that are not susceptible to the generation of mutations that cause a conditional phenotype will not be identified in such screens. To identify a more comprehensive collection of mutants, we used transposon mutagenesis to generate a large collection of lethal disruption mutations. This collection was used to identify 209 mutants with disruptions that cause an aberrant terminal bud morphology. The disruption mutations in 33 of these mutants identify three previously uncharacterized genes as essential, and the mutant phenotypes suggest roles for their products in bud morphogenesis.