Adhesion of human skin fibroblasts. Effect of tetravalent, divalent and monovalent concanavalin A.
Adhesion of human skin fibroblasts. Effect of tetravalent, divalent and monovalent concanavalin A.
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人皮肤成纤维细胞的粘附。
DOI：
10.1016/0014-4827(78)90210-0
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发表时间：
1978
影响因子：
3.7
通讯作者：
K. von Figura
中科院分区：
文献类型：
--
作者：
R. Prinz;K. von Figura
刀豆蛋白A （ConA）预处理抑制成纤维细胞对塑料表面的粘附呈剂量依赖性。ConA效应是可逆的，α-甲基甘露糖甙可以抑制ConA效应。细胞松弛素B （CB）和秋水仙碱预处理提高了ConA的作用。二价和单价ConA衍生物对成纤维细胞粘附无影响。这表明配体与ConA受体的附着不足以阻止细胞粘附。四价ConA对抑制细胞粘附的要求表明，膜组分横向迁移率的降低是抑制细胞粘附的原因，这似乎是四价ConA所特有的。秋水仙碱和炭黑预处理对ConA作用的增强表明微管和微丝参与了成纤维细胞ConA受体的迁移。
Concanavalin A (ConA) pretreatment inhibited the adhesion of fibroblasts to plastic surface in a dose-dependent manner. The ConA effect was reversible and could be inhibited by α-methylmannoside. Pretreatment with cytochalasin B (CB) and colchicine increased the ConA effect. Divalent and monovalent ConA derivatives had no effect on the fibroblast adhesion. This indicates that ligand attachment to ConA receptors is not sufficient to prevent cell adhesion. The requirement of tetravalent ConA for inhibition of cell adhesion suggests that the decrease of lateral mobility of membrane components, which seems to be specific for tetravalent ConA, is responsible for the inhibition of cell adhesion. The enhancement of the ConA effect by colchicine and CB pretreatment suggests an involvement of microtubules and microfilaments in the mobility of ConA receptors of fibroblasts.