EXPRESSION AND LOCALIZATION OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR MESSENGER-RNA AND ITS PROTEIN IN RAT-BRAIN
EXPRESSION AND LOCALIZATION OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR MESSENGER-RNA AND ITS PROTEIN IN RAT-BRAIN
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DOI：
10.1172/jci118080
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发表时间：
1995-07-01
影响因子：
15.9
通讯作者：
BROUSSARD, DL
中科院分区：
文献类型：
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作者：
MULBERG, AE;RESTA, LP;BROUSSARD, DL
在以前的研究中，我们的特点是表达的囊性纤维化跨膜传导调节（CFTR）蛋白网格蛋白包被的囊泡来自牛脑和大鼠脑神经元。本研究进一步用逆转录聚合酶链反应扩增（RT-PCR）、原位杂交和免疫细胞化学等方法对大鼠脑内CFTR蛋白mRNA和蛋白的表达进行了研究。结果表明，CFTR mRNA和蛋白在参与食欲和静息能量消耗调节的下丘脑、丘脑和杏仁核等脑区的表达是一致的。CFTR的存在下，定位于这些地区的大脑控制稳态和能量消耗的神经元可能阐明其他非肺和胃肠道表现，通常是在儿童囊性纤维化的发病机制。脑内突变CFTR对正常神经肽囊泡运输的失调可能是破坏体内平衡的致病机制。
In previous studies we have characterized the expression of the cystic fibrosis transmembrane conductance regulator (CFTR) protein in clathrin-coated vesicles derived from bovine brain and in neurons of rat brain. In this study we have further characterized the expression of the CFTR protein mRNA and protein in rat brain with reverse transcriptase polymerase chain reaction amplification (RT-PCR), in situ hybridization, and immunocytochemistry, The expression of CFTR mRNA and protein in discrete areas of brain, including tbe hypothalamus, thalamus, and amygdaloid nuclei, which are involved in regulation of appetite and resting energy expenditure, is identical. The presence of CFTR in neurons localized to these regions of brain controlling homeostasis and energy expenditure may elucidate the pathogenesis of other nonpulmonary and gastrointestinal manifestations which commonly are observed in children with cystic fibrosis. Dysregulation of normal neuropeptide vesicle trafficking by mutant CFTR in brain may serve as a pathogenic mechanism for disruption of homeostasis.