Immune enhancement assessment of dietry incorporated marine alga Sargassum wightii (Phaeophyceae/Punctariales) in tiger shrimp Penaeus monodon (Crustacia/Penaeidae) through prophenoloxidase (proPO) systems
Immune enhancement assessment of dietry incorporated marine alga Sargassum wightii (Phaeophyceae/Punctariales) in tiger shrimp Penaeus monodon (Crustacia/Penaeidae) through prophenoloxidase (proPO) systems
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发表时间：
2004-12
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通讯作者：
S. Felix;P. Robins;A. Rajeev
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作者：
S. Felix;P. Robins;A. Rajeev
通过为期30 d的试验，研究了海藻卫氏马尾藻对斑节对虾的免疫增强效果。用不同浓度的褐藻S.分别为10、20和30 g/kg。用海洋病原体副溶血性弧菌对它们进行挑战，以评估由于免疫增强而导致的存活率的增加。另一组虾用于抽取血淋巴以估计酚氧化酶原活性水平的增加。以3天即1、3、6、9和12天的定期间隔进行用于上述分析的取样。试验饲料（10 g/kg）组在12 d时酚氧化酶原活性最高（0.62），成活率最高（83%）。因此，10 g/kg的S.添加到饮食中的wightii可能是一种对对虾无害且经济可行的免疫刺激剂。
An experiment of 30 days duration was conducted to test the efficacy of the seaweed Sargassum wightii, as immunostimulant in tiger shrimp Penaeus monodon. The shrimps were fed with the experimental diets coated with different concentrations of the brown algae S. wightii viz. 10, 20 and 30 g/kg. They were challenged with a marine pathogen Vibrio parahaemolyticus to assess the increase in survival rate if any due to immune enhancement. Another group of shrimp was used for drawing the haemolymph to estimate the increase in the level of prophenoloxidase activity. Samplings for the above said analysis were carried out at regular intervals of 3 days viz. 1, 3, 6, 9 and 12 days. The highest prophenoloxidase activity (0.62) and the highest survival rate (83%) were recorded on the 12 day with the experimental diet (10 g/kg) . Hence the 10 g/kg of S. wightii added to the diet could be an eco-friendly and economically viable immunostimulant for penaeid shrimps.