Comparison and combination of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study.
Comparison and combination of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study.
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DOI：
10.1364/boe.8.004629
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发表时间：
2017-09
影响因子：
3.4
通讯作者：
Chen Wu;Henry Le;Shihao Ran;Manmohan Singh;I. Larina;D. Mayerich;M. Dickinson;K. Larin
中科院分区：
文献类型：
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作者：
Chen Wu;Henry Le;Shihao Ran;Manmohan Singh;I. Larina;D. Mayerich;M. Dickinson;K. Larin
几种光学成像技术已被应用于高分辨率胚胎成像使用不同的对比度机制，每个都有自己的优点和局限性。在这项研究中，我们成像相同的E9.5小鼠胚胎旋转成像光学相干断层扫描（RI-OCT）和选择性平面照明显微镜（SPIM）。RI-OCT通过从多个角度对样本进行成像，克服了传统OCT成像的光学穿透限制，该限制禁止在后期阶段对小鼠胚胎进行全身成像。SPIM通过用聚焦的光片照射样品，实现了比激光扫描共聚焦显微镜（LSCM）更少光毒性和光漂白的高分辨率3D成像。并排比较补充了共同注册的图像。结果表明，SPIM和RI-OCT具有很强的互补性，可以为小鼠胚胎研究提供更全面的组织定征。
Several optical imaging techniques have been applied for high-resolution embryonic imaging using different contrast mechanisms, each with their own benefits and limitations. In this study, we imaged the same E9.5 mouse embryo with rotational imaging optical coherence tomography (RI-OCT) and selective plane illumination microscopy (SPIM). RI-OCT overcomes optical penetration limits of traditional OCT imaging that prohibit full-body imaging of mouse embryos at later stages by imaging the samples from multiple angles. SPIM enables high-resolution, 3D imaging with less phototoxicity and photobleaching than laser scanning confocal microscopy (LSCM) by illuminating the sample with a focused sheet of light. Side by side comparisons are supplemented with co-registered images. The results demonstrate that SPIM and RI-OCT are highly complementary and could provide more comprehensive tissue characterization for mouse embryonic research.