The Effect of Transcatheter Injections on Cell Viability and Cytokine Release of Mononuclear Cells
The Effect of Transcatheter Injections on Cell Viability and Cytokine Release of Mononuclear Cells
复制标题
DOI：
10.3174/ajnr.a2092
复制
发表时间：
2010-09-01
影响因子：
3.5
通讯作者：
Savitz, S. I.
中科院分区：
文献类型：
--
作者：
El Khoury, R.;Misra, V.;Savitz, S. I.
背景和目的：几项研究表明，各种类型的细胞疗法可以促进动物模型中风后的恢复。IA为基础的交付的细胞到大脑正在调查中风，但它是未知的细胞是否受伤的结果，通过导管注射或暴露于碘造影剂或含有heparin.MATERIALS的解决方案：我们评估的影响导管与Excelsior SL-10导管或暴露于肝素或碘造影剂对人骨髓MNCs。通过台盼蓝拒染法、半胱天冬酶-3活性和脂质过氧化来评估细胞活力和细胞损伤。结果：1000万个细胞的流速在0.5 ~ 2 mL/min之间并不改变MNC的存活率，但5 mL/min的流速使MNC的存活率降低了19%。碘和低剂量肝素暴露不影响细胞活力;然而，高剂量肝素具有细胞毒性。2 mL/min的导管输送不影响VEGF、IL-10或IGF-1的水平。结论：在流速高达2 mL/min时，肝素、碘造影剂和Excelsior SL-10导管似乎不会损伤MNC。然而，较高流速确实会降低存活率，高剂量肝素确实会导致细胞死亡。
BACKGROUND AND PURPOSE: Several studies suggest that various types of cellular therapies enhance recovery after stroke in animal models. IA-based delivery of cells to the brain is under investigation for stroke, but it is unknown whether cells are injured as a result of being injected through a catheter or exposed to iodinated contrast medium or solutions containing heparin.MATERIALS AND METHODS: We assessed the effect of catheterization with the Excelsior SL-10 catheter or exposure to heparin or iodine contrast on human bone marrow MNCs. Viability and cell injury were assessed by trypan blue exclusion, caspase-3 activity, and lipid peroxidation. Cellular function of MNCs was assessed by their production and release of VEGF, IL-10, and IGF-1.RESULTS: Flow rates of 10 million cells from 0.5 to 2 mL/min did not alter MNC viability; however, 5 mL/min of MNCs did reduce viability by 19%. Iodine and low-dose heparin exposure did not affect cell viability; however, high-dose heparin was cytotoxic. Catheter delivery at 2 mL/min did not affect levels of VEGF, IL-10, or IGF-1.CONCLUSIONS: MNCs do not appear to be damaged by heparin, iodine contrast, and the Excelsior SL-10 catheter at flow rates up to 2 mL/min. However, higher flow rates did reduce viability, and high-dose heparin did cause cell death.