The Salicylic Acid Signaling Pathway Plays an Important Role in the Resistant Process of Brassica rapa L. ssp. pekinensis to Plasmodiophora brassicae Woronin
The Salicylic Acid Signaling Pathway Plays an Important Role in the Resistant Process of Brassica rapa L. ssp. pekinensis to Plasmodiophora brassicae Woronin
复制标题
DOI：
10.1007/s00344-020-10105-4
复制
发表时间：
2020-04
影响因子：
4.8
通讯作者：
R. Ji;Shi-qi Gao;Q. Bi;Yilian Wang;Mingcan Lv;Wenjie Ge;H. Feng
中科院分区：
文献类型：
--
作者：
R. Ji;Shi-qi Gao;Q. Bi;Yilian Wang;Mingcan Lv;Wenjie Ge;H. Feng
根肿病是由根肿病菌（Plasmodiophora brassicaeWoronin）侵染十字花科蔬菜引起的一种病害，严重影响十字花科蔬菜的产量和经济效益。然而，P. brassicae和大白菜（Brassica rapaL.）北京亚种（ssp.pekinensis））仍然未知。本研究采用双向电泳技术筛选根肿病与对照根中差异表达蛋白。共有21个DEP在患病根中变化超过两倍，其中16个使用基质辅助激光解吸/电离飞行时间/质谱法成功鉴定。实时荧光定量PCR分析表明，16个候选基因中的大部分基因具有一致的转录和蛋白水平表达。基因本体分析表明，16个候选基因中有10个对刺激有反应。其中两个基因参与水杨酸（SA）信号通路。SA处理后，病根中SA含量和SA信号途径基因的表达发生变化，抗病性增强。因此，大白菜与P.芸苔属估计SA信号通路。本研究结果有助于提高大白菜根肿病的抗性。
Clubroot disease, caused byPlasmodiophora brassicaeWoronin infection, leads to significant yield and economic losses in cruciferous vegetables. However, the molecular mechanism underlying the interaction betweenP. brassicaeand Chinese cabbage (Brassica rapaL. ssp.pekinensis) remains unknown. In this study, two-dimensional electrophoresis was used to screen differentially expressed proteins (DEPs) in clubroot-diseased and control roots of Chinese cabbage. A total of 21 DEPs changed by more than twofold in the diseased roots, of which 16 were successfully identified using matrix-assisted laser desorption/ionization-time of flight/mass spectrometry. Quantitative real-time polymerase chain reactions’ analysis showed that most of the 16 candidate genes had the consistent transcription and protein level expression. Gene ontology analysis revealed that 10 out of 16 candidate genes responded to stimulus. Two of these genes were involved in the salicylic acid (SA) signaling pathway. The content of SA and the expression of genes in the SA signaling pathway were altered in the diseased roots and disease resistance increased after SA treatment. Thus, the interactions between Chinese cabbage andP. brassicaestimulate the SA signaling pathway. Our findings may contribute to improving clubroot resistance in Chinese cabbage.