Smart fluorescent probes for in situ imaging of enzyme activity: design strategies and applications.
Smart fluorescent probes for in situ imaging of enzyme activity: design strategies and applications.
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DOI：
10.4155/fmc-2018-0193
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发表时间：
2018-12
影响因子：
4.2
通讯作者：
Han Wei;Guanghua Wu;X. Tian;Ziwei Liu
中科院分区：
文献类型：
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作者：
Han Wei;Guanghua Wu;X. Tian;Ziwei Liu
酶在生命系统的生理和病理过程中起着关键作用。为了在分子和细胞水平上提供酶活性的详细图像，化学和生物学的跨学科研究导致了许多智能荧光探针的出现，这些探针仅在与其靶标相互作用时发射荧光或显示移位信号。与传统的“始终开启”探针相比，智能荧光探针具有更高的信噪比的明显优势，能够灵敏地检测具有临床意义的酶。在这篇综述中，我们总结了智能荧光探针的设计策略和选择的应用程序，在原位成像的酶活性。目前的挑战和未来的发展，在这一领域进行了讨论。
Enzymes play critical roles in the physiological and pathological processes of living systems. To provide detailed pictures of enzyme activity at the molecular and cellular levels, interdisciplinary studies of chemistry and biology have led to the emergence of many smart fluorescent probes, which emit fluorescence or show a shifted signal only upon interaction with their targets. With distinct advantage of a higher signal-to-noise ratio than traditional 'always on' probes, smart fluorescent probes enable sensitive detection of enzymes with clinical significance. In this review, we summarize the design strategies and selected applications of smart fluorescent probes for in situ imaging of enzyme activity. Current challenges and future developments in this field are also discussed.