Effect of alkylation with streptozotocin on the secondary structure of DNA
Effect of alkylation with streptozotocin on the secondary structure of DNA
复制标题
链脲佐菌素烷基化对 DNA 二级结构的影响
作者：
R. Rizvi;Shahabuddin;A. Rahman;S. M. Hadi
用S1核酸酶水解和羟基磷灰石层析法研究了烷基化抗生素链脲霉素对DNA二级结构的影响。小牛胸腺DNA在体外用浓度递增的链脲佐菌素烷基化，并进行S1核酸酶水解。观察到DNA降解程度增加，表明二级结构不稳定。间接的证据，推导出从碱水解，NaCl对S1核酸酶水解的影响，和羟基磷灰石色谱分析烷基化的DNA，提出了一个显着的烷基化的DNA磷酸除了DNA碱基。据报道，烟酰胺可改变链脲佐菌素的细胞毒性和致癌作用。我们的实验表明，在烟酰胺的存在下，链脲佐菌素导致形成的烷基化碱的比例更大的烷基磷酸三酯。这可能与烟酰胺存在和不存在时链脲佐菌素的细胞毒性差异有关。
S1 nuclease hydrolysis and hydroxyapatite chromatography were used to study the effect of the alkylating antibiotic, streptozotocin, on the secondary structure of DNA. Native calf thymus DNA was alkylatedin vitro with increasing concentrations of streptozotocin and subjected to S 1 nuclease hydrolysis. An increasing degree of DNA degradation was seen, suggesting a destabilization of the secondary structure. Indirect evidence, deduced from alkaline hydrolysis, effect of NaCl on S1 nuclease hydrolysis, and hydroxyapatite chromatographic analysis of alkylated DNA, suggested a significant alkylation of DNA phosphates in addition to DNA bases. Nictotinamide has been reported to alter the cytotoxic and carcinogenic effects of streptozotocin. Our experiments indicate that in the presence of nicotinamide, streptozotocin causes the formation of a greater proportion of alkylated bases in relation to alkyl phosphotriesters. This may have significance in relation to the differential cytotoxicity of streptozotocin in the absence and presence of nicotinamide.
用 N-甲基-N-亚硝基脲和 N-乙基-N-亚硝基脲对分离的染色质进行烷基化。
影响因子：
5.1
作者：
Marushige,K;Marushige,Y
通讯作者：
Marushige,Y