MicroRNA profiling in denatured dermis f deep burn patients
MicroRNA profiling in denatured dermis f deep burn patients
复制标题
深度烧伤患者变性真皮中的 MicroRNA 分析
DOI：
10.1016/j.burns.2011.10.014
复制
发表时间：
2012-06-01
期刊：
影响因子：
2.7
通讯作者：
Huang, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Liang, Pengfei;Lv, Chunliu;Huang, Xiaoyuan
背景：变性真皮是深度烧伤创面真皮的一部分，具有恢复正常形态和功能的能力。保留变性真皮的皮肤移植术是一种新的外科手术方法，临床效果满意，如减少瘢痕挛缩，更好地恢复外观和功能。然而，变性真皮功能恢复的潜在机制仍不清楚。microRNAs（miRNAs）是一类新的调控性非编码单链RNA，在正常发育和生理以及疾病发展中起着关键作用。本研究分析了患者变性真皮中miRNAs的表达谱，并进一步研究了miRNAs在功能恢复中的可能作用。方法：收集变性真皮和配对的正常皮肤标本，采用miRNA芯片技术进行分析。结果：与正常皮肤相比，变性真皮组织中有66条miRNA表达差异，其中34条下调，32条上调。其中上调最明显的是miR-663，下调最明显的是miR-203。结论：本研究发现的差异表达的miRNA为进一步了解变性真皮功能恢复的机制提供了实验依据。(C)2011 Elsevier Ltd和ISBI。All rights reserved.
Background: Denatured dermis is a part of the dermis in deep burn wound and has the ability to restore normal morphology and function. Skin grafting with the preservation of denatured dermis is a new kind of surgical procedure and has reported satisfactory clinical effects, such as lessened scar contracture and a better restoration of the appearance and function. However, the underlying mechanism of the recovery of denatured dermal function remains unclear. MicroRNAs (miRNAs) are a new class of regulatory noncoding single-stranded RNAs, which play a key role in normal development and physiology, as well as in disease development. This study analysed the profile of miRNAs in denatured dermis from patients and further investigated the possible roles of miRNAs played in the functional recovery. of denatured dermis by prediction of the potential target genes of differentially expressed miRNAs.Methods: The denatured dermis and paired normal skin were collected and analysed by miRNA array. The miRNA profiling results were validated by real-time reverse transcriptase polymerase chain reaction (RT-PCR), and bioinformatics' analysis was employed to further predict the miRNA targets.Results: A total of 66 miRNAs were differentially expressed in denatured dermis compared with those in normal skin, among which 34 were down-regulated while 32 are up-regulated. The most significantly up-regulated miRNA was miR-663, and the most significantly down-regulated one was miR-203. Differentially expressed miRNAs were predicted to be related with several signalling pathways in wound healing.Conclusion: The differential miRNA expression identified in this study supplies experimental basis for further understanding the mechanisms of functional recovery of the denatured dermis. (C) 2011 Elsevier Ltd and ISBI. All rights reserved.