A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome.
A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome.
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DOI：
10.1038/srep31731
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发表时间：
2016-08-25
影响因子：
4.6
通讯作者：
Jones MB
中科院分区：
文献类型：
--
作者：
Anderson EL;Li W;Klitgord N;Highlander SK;Dayrit M;Seguritan V;Yooseph S;Biggs W;Venter JC;Nelson KE;Jones MB
随着有关微生物和宿主之间可能关联的报道越来越多，对这些信息的更有意义的解释需要有能力比较不同研究的数据集。这取决于工作流程的标准化，以确保研究内部和研究之间的可比性。在这里，我们建议标准使用另一种收集和稳定方法，以便于进行这种比较。将DNA gentek OMNI基因∙肠道微生物组试剂盒与目前公认的冷冻社区标准进行比较，以便在进行微生物组研究的全基因组测序之前存储人类粪便样本。这种稳定和收集设备允许环境温度存储、自动化和样品运输/转移的简便性。该装置允许与冷冻样品相同的数据重复性，并产生更高的核酸回收率。使用DNA gentek采集设备采集和稳定粪便微生物样本，结合我们的提取和WGS，可提供强大、可重复的工作流程，为微生物组研究的粪便标准化全球采集、存储和分析提供支持。
As reports on possible associations between microbes and the host increase in number, more meaningful interpretations of this information require an ability to compare data sets across studies. This is dependent upon standardization of workflows to ensure comparability both within and between studies. Here we propose the standard use of an alternate collection and stabilization method that would facilitate such comparisons. The DNA Genotek OMNIgene∙Gut Stool Microbiome Kit was compared to the currently accepted community standard of freezing to store human stool samples prior to whole genome sequencing (WGS) for microbiome studies. This stabilization and collection device allows for ambient temperature storage, automation, and ease of shipping/transfer of samples. The device permitted the same data reproducibility as with frozen samples, and yielded higher recovery of nucleic acids. Collection and stabilization of stool microbiome samples with the DNA Genotek collection device, combined with our extraction and WGS, provides a robust, reproducible workflow that enables standardized global collection, storage, and analysis of stool for microbiome studies.