A COMPARISON OF GENE ORGANIZATION IN THE ZWF REGION OF THE GENOMES OF THE CYANOBACTERIA SYNECHOCOCCUS SP PCC-7942 AND ANABAENA SP PCC-7120
A COMPARISON OF GENE ORGANIZATION IN THE ZWF REGION OF THE GENOMES OF THE CYANOBACTERIA SYNECHOCOCCUS SP PCC-7942 AND ANABAENA SP PCC-7120
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DOI：
10.1111/j.1574-6968.1995.tb07882.x
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发表时间：
1995-11-01
影响因子：
2.1
通讯作者：
MANN, NH
中科院分区：
文献类型：
--
作者：
NEWMAN, J;KARAKAYA, H;MANN, NH
在单细胞蓝细菌聚球藻属PCC 7942和丝状异囊蓝细菌鱼腥藻属PCC 7120中分析了编码葡萄糖-6-磷酸脱氢酶基因zwf的基因组区域。蓝藻zwf序列的比较显示存在两个绝对保守的半胱氨酸残基，这可能是牵连在光/暗控制酶的活性。在两种菌株中存在的基因fbp，编码果糖-1，6-二磷酸酶，在zwf的上游，强烈表明，在这些生物体中的氧化戊糖磷酸途径可能会发挥作用，完全氧化葡萄糖6-磷酸为CO2。果糖-1，6-二磷酸酶的氨基酸序列不支持其通过巯基/二硫键交换机制进行光活化的想法。在鱼腥藻PCC 7120的情况下，编码转醛醇酶的tal基因位于zwf和fbp之间。
The region of the genome encoding the glucose-6-phosphate dehydrogenase gene zwf was analysed in a unicellular cyanobacterium, Synechococcus sp. PCC 7942, and a filamentous, heterocystous cyanobacterium, Anabaena sp. PCC 7120. Comparison of cyanobacterial zwf sequences revealed the presence of two absolutely conserved cysteine residues which may be implicated in the light/dark control of enzyme activity. The presence in both strains of a gene fbp, encoding fructose-1,6-bisphosphatase, upstream from zwf strongly suggests that the oxidative pentose phosphate pathway in these organisms may function to completely oxidize glucose 6-phosphate to CO,. The amino acid sequence of fructose-1,6-bisphosphatase does not support the idea of its light activation by a thiol/disulfide exchange mechanism. In the case of Anabaena sp. PCC 7120, the tal gene, encoding transaldolase, lies between zwf and fbp.