Real-Time IR Tracking of Single Reflective Micromotors through Scattering Tissues
Real-Time IR Tracking of Single Reflective Micromotors through Scattering Tissues
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DOI：
10.1002/adfm.201905272
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发表时间：
2019-10-21
影响因子：
19
通讯作者：
Schmidt, Oliver G.
中科院分区：
文献类型：
--
作者：
Aziz, Azaam;Medina-Sanchez, Mariana;Schmidt, Oliver G.
医用微电机有可能导致未来生物医学的范式转变，因为它们可以以微创方式进行主动药物输送，显微外科手术，组织工程或辅助受精。然而，翻译到临床治疗是具有挑战性的，因为许多应用的单个或几个微电机需要实时跟踪和控制在深组织中的高时空分辨率。虽然光学技术是这项任务的流行选择，但吸收和强光散射会导致信噪比随着穿透深度的增加而显著降低。在这里，介绍了一种高反射微电机，它反射的光强度是简单金粒子的十倍以上，并且可以通过外部磁场精确导航。一个定制的光学红外成像设置和图像相关技术的实施，以跟踪单微电机的实时和无标签的下幻影和离体小鼠颅骨组织。作为一个潜在的应用，微电机的速度被记录时，移动通过不同的粘性流体，以确定不同的生理流体的粘度，以远程心血管疾病的诊断。此外，微型马达装载有模型药物以证明其货物运输能力。所提出的反射式微马达适合作为用于皮下或器官芯片应用的治疗诊断工具。
Medical micromotors have the potential to lead to a paradigm shift in future biomedicine, as they may perform active drug delivery, microsurgery, tissue engineering, or assisted fertilization in a minimally invasive manner. However, the translation to clinical treatment is challenging, as many applications of single or few micromotors require real-time tracking and control at high spatiotemporal resolution in deep tissue. Although optical techniques are a popular choice for this task, absorption and strong light scattering lead to a pronounced decrease of the signal-to-noise ratio with increasing penetration depth. Here, a highly reflective micromotor is introduced which reflects more than tenfold the light intensity of simple gold particles and can be precisely navigated by external magnetic fields. A customized optical IR imaging setup and an image correlation technique are implemented to track single micromotors in real-time and label-free underneath phantom and ex vivo mouse skull tissues. As a potential application, the micromotors speed is recorded when moving through different viscous fluids to determine the viscosity of diverse physiological fluids toward remote cardiovascular disease diagnosis. Moreover, the micromotors are loaded with a model drug to demonstrate their cargo-transport capability. The proposed reflective micromotor is suitable as theranostic tool for sub-skin or organ-on-a-chip applications.