Membrane association of a nonconserved viral protein confers virus ability to extend its host range
Membrane association of a nonconserved viral protein confers virus ability to extend its host range
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DOI：
10.1016/j.virol.2015.03.047
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发表时间：
2015-08-01
期刊：
影响因子：
3.7
通讯作者：
Folimonova, Svetlana Y.
中科院分区：
文献类型：
--
作者：
Kang, Sung-Hwan;Bak, Aurelie;Folimonova, Svetlana Y.
柑橘衰退病毒(Citrus Tristeza Virus，CTV)是环状病毒科中最大、最复杂的成员，它编码一种独特的蛋白p33，该蛋白与其他已知蛋白没有同源性，但在病毒的致病过程中起着重要的作用。在这项研究中，我们研究了p33的一些特征。我们证明p33是一种膜相关蛋白，它通过蛋白质C末端疏水氨基酸残基形成的跨膜螺旋插入到膜上。该跨膜结构域(TMD)的去除显著改变了p33在细胞内的定位。此外，仅TMD一项就足以提供无关蛋白质的膜定位。最后，产生缺失TMD的截短p33的CTV变异体不能感染所选病毒宿主之一的酸橙，而感染需要p33，这表明p33的膜结合对于CTV扩大宿主范围的能力是重要的。(C)2015 Elsevier Inc.保留所有权利。
Citrus tristeza virus (CTV), the largest and most complex member of the family Closteroviridae, encodes a unique protein, p33, which shows no homology with other known proteins, however, plays an important role in virus pathogenesis. In this study, we examined some of the characteristics of p33. We show that p33 is a membrane-associated protein that is inserted into the membrane via a transmembrane helix formed by hydrophobic amino acid residues at the C-terminal end of the protein. Removal of this transmembrane domain (TMD) dramatically altered the intracellular localization of p33. Moreover, the TMD alone was sufficient to confer membrane localization of an unrelated protein. Finally, a CTV variant that produced a truncated p33 lacking the TMD was unable to infect sour orange, one of the selected virus hosts, which infection requires p33, suggesting that membrane association of p33 is important for the ability of CTV to extend its host range. (C) 2015 Elsevier Inc. All rights reserved.