Comparative transcriptome and metabolomic profiling reveal the complex mechanisms underlying the developmental dynamics of tobacco leaves
Comparative transcriptome and metabolomic profiling reveal the complex mechanisms underlying the developmental dynamics of tobacco leaves
复制标题
比较转录组和代谢组学分析揭示了烟叶发育动态背后的复杂机制
DOI：
10.1016/j.ygeno.2020.07.005
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发表时间：
2020-11-01
期刊：
影响因子：
4.4
通讯作者：
Lu, Kun
中科院分区：
文献类型：
--
作者：
Chang, Wei;Zhao, Huina;Lu, Kun
虽然叶片是大多数植物最重要的光合器官，但叶片发育动态的许多分子机制仍有待探索。为了更好地了解参与叶片发育的转录调控机制，我们进行了比较转录组学和代谢组学分析的叶片从7个位置上的烟草（烟草）植物。共鉴定了35，622个独特的差异表达基因和79种代谢产物。时间序列表达分析检测到两个有趣的转录谱，一个包含10,197个基因，在叶发育过程中显示持续上调，另一个包含4696个基因，显示持续下调。结合这些数据与共表达网络的结果确定了四个重要的调控网络参与光呼吸和三羧酸循环，这些网络可以调节叶片发育过程中的碳/氮平衡。我们还发现，转录因子NtGATA 5作为一个枢纽与C和N代谢和叶绿体发育过程中，通过调节植物激素。此外，我们还研究了烟草叶片发育过程中生长素、细胞分裂素和茉莉酸生物合成及信号通路相关基因的转录动态。总的来说，我们的研究大大扩展了对控制植物叶片发育动态的调控网络的理解。
Although the leaf is the most important photosynthetic organ in most plants, many of the molecular mechanisms underlying leaf developmental dynamics remain to be explored. To better understand the transcriptional regulatory mechanisms involved in leaf development, we conducted comparative transcriptomic and metabolomic analysis of leaves from seven positions on tobacco (Nicotiana tabacum) plants. A total of 35,622 unique differentially expressed genes and 79 metabolites were identified. A time-series expression analysis detected two interesting transcriptional profiles, one comprising 10,197 genes that displayed continual up-regulation during leaf development and another comprising 4696 genes that displayed continual down-regulation. Combining these data with co-expression network results identified four important regulatory networks involved in photorespiration and the tricarboxylic acid cycle; these networks may regulate carbon/nitrogen balance during leaf development. We also found that the transcription factor NtGATA5 acts as a hub associated with C and N metabolism and chloroplast development during leaf development through regulation of phytohormones. Furthermore, we investigated the transcriptional dynamics of genes involved in the auxin, cytokinin, and jasmonic acid biosynthesis and signaling pathways during tobacco leaf development. Overall, our study greatly expands the understanding of the regulatory network controlling developmental dynamics in plant leaves.