DNA-controlled assembly of soft nanoparticles
DNA-controlled assembly of soft nanoparticles
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DOI：
10.1021/ja8030054
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发表时间：
2008-08-13
影响因子：
15
通讯作者：
Vogel, Stefan
中科院分区：
文献类型：
--
作者：
Jakobsen, Ulla;Simonsen, Adam C.;Vogel, Stefan
将 DNA（编码）固定在固体纳米粒子上需要表面化学，这对于金表面来说是很成熟的，但通常很繁琐，并且通常不适用于许多其他无机表面材料。虽然人们在扩展固体纳米粒子的表面化学技术方面投入了大量精力，但对 DNA 与软纳米粒子（如脂质体）非共价连接的开发的关注却少之又少。在这里，我们报道了溶液中和固体支撑膜上的 DNA 控制的脂质体组装，该过程显示出从组装状态到分解状态的非常急剧的热转变，允许应用 DNA 控制的脂质体组装来检测具有单错配辨别能力的多核苷酸（例如 DNA）。该方法基于单条 DNA 链（包含两个脂质膜锚定点），它能够非共价附着到脂质体表面。该设计能够检测生物多核苷酸靶标，因为互补链可以是未修饰的 DNA 和 RNA 链。
Immobilization of DNA (encoding) on solid nanoparticles requires surface chemistry, which is well established for gold surfaces but often tedious and not generally applicable for many other inorganic surface materials. While substantial effort has been devoted to expanding surface chemistry techniques for solid nanoparticles, considerably less attention has been given to the development of noncovalent attachment of DNA to soft nanoparticles, like liposomes. Here we report a DNA-controlled assembly of liposomes in solution and on solid supported membranes, this process displays remarkably sharp thermal transitions from an assembled to a disassembled state, allowing application of DNA-controlled liposome assembly for the detection of polynucleotides (e.g., DNA) with single mismatch discrimination power. The method is based on a single DNA strand (contains two lipid membrane anchors), which is able to noncovalently attach to a liposome surface. This design enables detection of biological polynucleotide targets as the complementary strand can be unmodified DNA and RNA strands.