miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro.
miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro.
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miR-132体外通过靶标MMP16抑制胶质瘤细胞侵袭和迁移
DOI：
10.2147/ott.s79282
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发表时间：
2015
影响因子：
4
通讯作者：
Zhou YX
中科院分区：
文献类型：
--
作者：
Wang H;Li XT;Wu C;Wu ZW;Li YY;Yang TQ;Chen GL;Xie XS;Huang YL;Du ZW;Zhou YX
胶质瘤是最常见的恶性原发性脑肿瘤，迫切需要新的临床生物标志物和治疗靶点。微小RNA（miRNA）是一类新型的小型非编码RNA（~ 22 nt），参与多种生物过程的调节。在这里，通过使用实时聚合酶链反应，发现miRNA-132在胶质瘤组织中显著失调。基于对miR-132靶基因的预测，我们假设miR-132与基质金属蛋白酶（MMP）16（MT3-MMP）（MMP家族的一种蛋白质）之间存在显著关联。我们发现miR-132的上调表达抑制了人脑胶质瘤细胞系A172、SHG 44和U87中的细胞迁移和侵袭。此外，miR-132的过表达降低了A172、SHG 44和U87细胞中MMP 16的表达。综上所述，我们的研究表明，miR-132影响胶质瘤细胞的迁移和MMP 16的侵袭，并暗示miR-132作为胶质瘤转移抑制miRNA。
Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas.