Live imaging of glioblastoma cells in brain tissue shows requirement of actin bundles for migration
Live imaging of glioblastoma cells in brain tissue shows requirement of actin bundles for migration
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DOI：
10.1017/s1740925x06000111
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发表时间：
2006-01-01
影响因子：
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通讯作者：
Small, J. Victor
中科院分区：
文献类型：
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作者：
Caspani, Elisabetta M.;Echevarria, Diego;Small, J. Victor
通过对转染了肌动蛋白细胞骨架标记物的胶质母细胞瘤U-373和U-87细胞的活细胞成像，研究了与二维和三维基质以及脑组织中的迁移相关的重排。在胶原凝胶和脑片中，两种类型的细胞都发育成神经元样突起，有褶皱的膜和丝状足突。也可以观察到起泡细胞，但这些细胞主要是静止的。在组织环境中，拖尾细胞突起的收缩与轴向应力纤维束的瞬时发展和收缩有关。Rho激酶的抑制导致脑片中的胶质母细胞瘤细胞不能移动，并随机地形成轴突，这表明Rho信号和收缩能力是迁移所必需的。在注射到活体小鼠脑内的胶质母细胞瘤细胞中也观察到了肌动蛋白应激纤维。因此，侵袭性胶质母细胞瘤细胞使用轴突样延伸穿透神经纤维和收缩肌动蛋白束之间，以牵引细胞体。
Live-cell imaging of glioblastoma U-373 and U-87 cells transfected with actin cytoskeleton markers has been used to study the re-arrangements that are associated with migration in two- and three-dimensional matrices and in brain tissue. In collagen gels and in brain slices, both cell types developed neuronal-like processes with ruffling membranes and filopodia. Blebbing cells were also observed, but these were mainly immobile. The retraction of trailing cell processes in a tissue environment was associated with the transient development and contraction of bundles of axial stress fibers. The inhibition of Rho-kinase caused glioblastoma cells in brain slices to become immobile and develop neurite-like processes at random, which indicates the requirement of Rho signaling and contractility for migration. Actin stress fibers were also observed in glioblastoma cells injected into the brains of living mice. Thus, invading glioblastoma cells use neurite-like extensions to penetrate between neuronal fibers and contractile actin bundles for traction of the cell body.