Candida rugosa lipase covalently immobilized on facilely-synthesized carbon nitride nanosheets as a novel biocatalyst.
Candida rugosa lipase covalently immobilized on facilely-synthesized carbon nitride nanosheets as a novel biocatalyst.
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假丝酵母脂肪酶共价固定在易于合成的氮化碳纳米片上作为新型生物催化剂
DOI：
10.1039/c8ra00536b
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发表时间：
2018-04-17
期刊：
影响因子：
3.9
通讯作者：
Shi, Jie
中科院分区：
文献类型：
--
作者：
Li, Ya;Ruan, Zhijun;Zheng, Mingming;Deng, Qianchun;Zhang, Shan;Zheng, Chang;Tang, Hu;Huang, Fenghong;Shi, Jie
我们报道了一种以氮化碳纳米片（C3N4 - NS）为基质的固定化皱褶假丝酵母脂肪酶的合理设计与制备。
The immobilization of lipase on solid supports provides a significant improvement to the stability and reusability of lipase. During immobilization, the restricted surface area and inferior separation capacity of matrix materials are crucial for obtaining high-quality immobilized lipase. Carbon nitride nanosheets (C3N4-NS) as a type of two-dimensional nanomaterial have attracted various attentions for their prominent 2D planar nanostructure, characteristic surface area, thermostability and biocompatibility. Herein, we report a rational design and fabrication of immobilized Candida rugosa lipase based on carbon nitride nanosheets (C3N4-NS) as the matrix. The synthetic C3N4-NS are characterized by transmission electron microscopy, Brunauer–Emmett–Teller gas sorptometry measurement, X-ray powder diffraction, Fourier transform infrared spectroscopy and thermogravimetric analysis. These results show that C3N4-NS possess an as-expected two-dimensional nanostructure with a large surface area of 74.374 m2 g−1. In addition, we chose glutaraldehyde-assisted covalent attachment to combine C3N4-NS and Candida rugosa lipase (CRL) via amino groups at the margins of C3N4-NS. The as-constructed immobilized lipase (C3N4-NS@CRL) exhibits satisfactory enzyme-loading (44.76 mg g−1), pH-flexibility, thermostability (after 180 min at 50 °C, 67% of the initial activity remained) and recyclability (after 10 runs, 72% of the initial activity remained). When compared with the free CRL, all experimental data indicate that C3N4-NS@CRL exhibited improved stability and enhanced practicability. To our knowledge, this is the first report of the application of carbon nitride nanosheets to enzyme immobilization.
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