Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's
Tryptic digest analysis by comprehensive reversed phase x two reversed phase liquid chromatography (RP-LC x 2RP-LC) at different pH's
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DOI：
10.1002/jssc.200800578
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Sandra, Pat
中科院分区：
文献类型：
--
作者：
Francois, Isabelle;Cabooter, Deirdre;Sandra, Pat
作为强阳离子交换SCX-LC和RP-LC结合分离复杂蛋白质组样品的经典方法的替代方案，本文描述了BSA和人血清的在线综合RP-LC x RP-LC分离。通过在两个维度上应用显著不同的pH，实现了高正交性和峰容量。熔融芯柱的串联耦合确保了第一维的高效率，而先前设计的具有平行第二维柱的接口进一步增强了综合系统的分离能力。
As all alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LC x RP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.