Identification and Expression Analysis of the PIN and AUX/LAX Gene Families in Ramie (Boehmeria nivea L. Gaud)
Identification and Expression Analysis of the PIN and AUX/LAX Gene Families in Ramie (Boehmeria nivea L. Gaud)
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苎麻 (Boehmeria nivea L. Gaud) PIN 和 AUX/LAX 基因家族的鉴定和表达分析
DOI：
10.3390/agronomy9080435
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发表时间：
2019-08-01
期刊：
影响因子：
3.7
通讯作者：
Peng, Dingxiang
中科院分区：
文献类型：
--
作者：
Bao, Yaning;Huang, Xing;Peng, Dingxiang
生长素调节生长和发育的各个方面。此外，生长素极性运输，这是由PIN形成（PIN）和AUX 1/LIKE AUX（AUX/LAX）蛋白介导的，在生长素分布中起着至关重要的作用。在本研究中，在苎麻（Boehmeria nivea L.）中鉴定了6个PIN和4个AUX/LAX基因。我们使用qRT-PCR来表征和分析这两个基因家族，包括系统发育关系、内含子/外显子结构、顺式元件、亚细胞定位以及在不同组织中的表达模式。这些基因的表达响应吲哚-3-乙酸（IAA）处理和干旱胁迫进行了评估，结果表明，大多数的BnAUX/LAX和BnPIN基因的调节作为结果IAA处理和干旱胁迫。本研究为深入了解苎麻生长素转运蛋白及其在苎麻纤维发育和逆境适应中的生物学功能奠定了基础。
Auxin regulates diverse aspects of growth and development. Furthermore, polar auxin transport, which is mediated by the PIN-FORMED (PIN) and AUXIN1/LIKE-AUX (AUX/LAX) proteins, plays a crucial role in auxin distribution. In this study, six PIN and four AUX/LAX genes were identified in ramie (Boehmeria nivea L.). We used qRT-PCR to characterize and analyze the two gene families, including phylogenetic relationships, intron/exon structures, cis-elements, subcellular localization, and the expression patterns in different tissues. The expression of these genes in response to indole-3-acetic acid (IAA) treatment and drought stress was also assessed; the results indicate that most of the BnAUX/LAX and BnPIN genes were regulated as a result of IAA treatment and drought stress. Our study provides insights into ramie auxin transporters and lays the foundation for further analysis of their biological functions in ramie fiber development and adaptation to environmental stresses.