Reactivity of monoclonal anti‐melanoma antibodies with melanoma cells freshly isolated from primary and metastatic melanoma
Reactivity of monoclonal anti‐melanoma antibodies with melanoma cells freshly isolated from primary and metastatic melanoma
复制标题
单克隆抗黑色素瘤抗体与从原发性和转移性黑色素瘤中新鲜分离的黑色素瘤细胞的反应性
DOI：
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发表时间：
1979
影响因子：
5.4
通讯作者：
W. Clark
中科院分区：
文献类型：
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作者：
Z. Steplewski;M. Herlyn;D. Herlyn;H. Koprowski;W. Clark
检测了从9例原发性和转移性黑素瘤病例新鲜获得的人黑素瘤细胞与杂交瘤克隆体外产生的单克隆抗黑素瘤抗体的结合。抗黑色素瘤单克隆抗体可与黑色素瘤细胞结合，但不与来自同一患者的非恶性细胞或来自巨大毛痣的细胞发生反应。这些结果证实存在肿瘤特异性抗原。单克隆抗体与来自不同患者的几种来源（原发性或转移性）的黑素瘤细胞的结合表明存在人黑素瘤细胞共有的肿瘤抗原。不同抗体与各种细胞的结合模式也预测了黑色素瘤细胞上存在一种以上的肿瘤特异性抗原决定簇。
Human melanoma cells, freshly obtained from nine primary and metastatic melanoma cases, were tested for binding of monoclonal anti‐melanoma antibodies produced in vitro by hybridoma clones. Monoclonal anti‐melanoma antibodies bind to melanoma cells but do not react with nonmalignant cells obtained from the same patients or with cells obtained from giant hairy nevus. These results confirm the existence of tumor‐specific antigens. Binding of monoclonal antibodies to melanoma cells of several origins, primary or metastatic, from different patients suggests the existence of tumor antigens shared by human melanoma cells. The binding pattern of different antibodies to various cells also predicts the existence of more than one tumor‐specific antigenic determinant on melanoma cells.