Increasing intracellular cAMP and cGMP inhibits cadmium-induced oxidative stress in rat submandibular saliva
Increasing intracellular cAMP and cGMP inhibits cadmium-induced oxidative stress in rat submandibular saliva
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DOI：
10.1016/s1532-0456(03)00120-0
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发表时间：
2003-07-01
影响因子：
3.9
通讯作者：
Zafari, K
中科院分区：
文献类型：
--
作者：
Abdollahi, M;Bahreini-Moghadam, A;Zafari, K
研究了镉对大鼠下颌下唾液氧化应激的诱导作用，以及特定磷酸二酯酶抑制剂、茶碱和西地那非增加细胞内cAMP和cGMP的保护作用。以匹罗卡品为促分泌剂，用微聚乙烯套管从麻醉大鼠口腔内收集颌下腺纯唾液。急性给药镉（10 mg/kg）通过增加脂质过氧化副产物（硫代巴比妥反应物质，TBARS）和降低唾液的总硫醇和总抗氧化能力引起显著的氧化应激。茶碱（25 mg/kg）和西地那非（5 mg/kg）同时治疗大鼠，可防止镉诱导的唾液氧化应激。茶碱和西地那非抑制镉诱导的脂质过氧化增加、总硫醇和抗氧化能力降低。由此可见，镉对大鼠下颌下唾液氧化应激的影响可通过同时给予特异性环核苷酸磷酸二酯酶抑制剂来保护。(C) 2003 Elsevier Inc.版权所有。
The effect of cadmium on induction of oxidative stress in rat submandibular saliva and protective role of increasing intracellular cAMP and cGMP by use of specific phosphodiesterase inhibitors, theophylline and sildenafil were investigated. Pure submandibular saliva was collected intraorally by micro polyethylene cannula from anaesthetized rats using pilocarpine as secretagogue. Acute administration of cadmium (10 mg/kg) caused significant oxidative stress by increasing lipid peroxidation by-products (thiobarbituric reactive substances, TBARS) and decreasing total thiols and total antioxidant power of the saliva. Concurrent therapy of rats by theophylline (25 mg/kg) and sildenafil (5 mg/kg) prevented cadmium-induced oxidative stress in saliva. Theophylline and sildenafil inhibited cadmium-induced increase in lipid peroxidation and decrease in total thiols and antioxidant power. It is concluded that cadmium administration results in oxidative stress in rat submandibular saliva, which can be protected by concurrent administration of specific cyclic nucleotide phosphodiesterase inhibitors. (C) 2003 Elsevier Inc. All rights reserved.