Cytoplasmic volume condensation is an integral part of mitosis
Cytoplasmic volume condensation is an integral part of mitosis
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DOI：
10.4161/cc.6.13.4357
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发表时间：
2007-07-01
期刊：
影响因子：
4.3
通讯作者：
Sontheimer, Harald
中科院分区：
文献类型：
--
作者：
Habela, Christa W.;Sontheimer, Harald
细胞生长和渗透体积调节无疑与细胞周期的锡永有关，因为每次分裂，新产生的细胞必须补偿其姐妹细胞一半的体积损失。然而，在哺乳动物细胞中，大小影响细胞周期决定的程度是有争议的。此外，细胞可以监测并因此调节其大小的机制尚未完全阐明。尽管一直存在争议，但很少有研究直接在单细胞真实的时间实验中解决这个问题。在这项研究中，我们使用荧光延时成像来定量评估在整个细胞周期中单个自发分裂细胞的体积。与生物物理学研究一起，这些研究确定，随着胶质瘤细胞通过有丝分裂的进展，盐和水的流出引起细胞质体积的浓缩。当细胞经历这种有丝分裂前浓缩（PMC）时，它们在每次分裂之前接近优选的细胞体积。这在功能上与染色质凝聚有关，表明PMC在有丝分裂中起着不可或缺的作用。
Cell growth and osmotic volume regulation are undoubtedly linked to the progres sion of the cell cycle as with each division, a newly generated cell must compensate for loss of half of its volume to its sister cell. The extent to which size influences cell cycle decisions, however, is controversial in mammalian cells. Further, a mechanism by which cells can monitor and therefore regulate their size has not been fully elucidated. Despite an ongoing debate, there have been few studies which directly address the question in single cell real - time experiments. In this study we used fluorescent time - lapse imaging to quantitatively assess volume in individual spontaneously dividing cells throughout the cell cycle. Together with biophysical studies, these establish that the efflux of salt and water brings about a condensation of cytoplasmic volume as glioma cells progress through mitosis. As cells undergo this pre -mitotic condensation ( PMC) they approach a preferred cell volume preceding each division. This is functionally linked to chromatin condensa tion, suggesting that PMC plays an integral role in mitosis.